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This compound is also unstable in alkaline solution when it rapidly forms a brown colouration it is therefore recommended that alkaline is not used to dissolve this compound.
We recommend stock solutions are made up in water, aliquoted immediately and stored at -20°C. They should be used within one week.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120007 in the following tested applications.
|Functional Studies||Use at an assay dependent concentration.|
SK-N-SH cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of (S)-3,5-DHPG (ab120007). Increased expression of CREB (phospho S133) in SK-N-SH cells correlates with an increase (S)-3,5-DHPG concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab32096 at 1/500 dilution and ab32515 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"