Overview

  • Product name(S)-3,5-DHPG
  • Description
    Selective group I mGlu agonist
  • Biological descriptionSelective group I mGlu agonist.

    Also available in simple stock solutions (ab144484) - add 1 ml of water to get an exact, ready-to-use concentration.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120007 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    (S)-3,5-DHPG images

    • SK-N-SH cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of (S)-3,5-DHPG (ab120007). Increased expression of CREB (phospho S133) in SK-N-SH cells correlates with an increase (S)-3,5-DHPG concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab32096 at 1/500 dilution and ab32515 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    References for (S)-3,5-DHPG (ab120007)

    This product has been referenced in:
    • Kew JN & Kemp JA Ionotropic and metabotropic glutamate receptor structure and pharmacology. Psychopharmacology (Berl) 179:4-29 (2005). Read more (PubMed: 15731895) »
    • Barton ME & Shannon HE Behavioral and convulsant effects of the (S) enantiomer of the group I metabotropic glutamate receptor agonist 3,5-DHPG in mice. Neuropharmacology 48:779-87 (2005). Read more (PubMed: 15829250) »
    • Wisniewski K & Car H (S)-3,5-DHPG: a review. CNS Drug Rev 8:101-16 (2002). Read more (PubMed: 12070529) »

    See all 15 Publications for this product

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    The Material Safety Data Sheets (MSDS) for our products that require one are located on the online product datasheet as a PDF file. For this product, ab120007, please see the PDF file at http://www.abcam.com/ps/Products/120/ab120007/Msds/ab12...

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    I have checked our internal datasheet and verified this product does not require any particular shipping temperature. If it was shipped with other products that needed to be stored at -4C, then blue ice would have...

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    Le laboratoire vient de m'informer que le poids moléculaire donné sur la fiche technique en ligne correspond à la structuredonnée pour ce composé.

    La diff&eacu...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"