Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146)

Overview

  • Product nameAnti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end
    See all S adenosylhomocysteine hydrolase primary antibodies
  • Description
    Rabbit polyclonal to S adenosylhomocysteine hydrolase - Aminoterminal end
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to N terminal residues of human Adenosylhomocysteine hydrolase

Properties

Applications

Our Abpromise guarantee covers the use of ab56146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • FunctionAdenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
  • PathwayAmino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
  • Involvement in diseaseDefects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
  • Sequence similaritiesBelongs to the adenosylhomocysteinase family.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosylhomocysteinase antibody
    • AdoHcyase antibody
    • ahcY antibody
    • S adenosyl L homocysteine hydrolase antibody
    • S adenosylhomocysteine hydrolase antibody
    • S-adenosyl-L-homocysteine hydrolase antibody
    • SAHH antibody
    • SAHH_HUMAN antibody
    see all

Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end images

  • All lanes : Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146) at 1 µg/ml + Recombinant Human S adenosylhomocysteine hydrolase protein (ab99326) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds

References for Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146)

ab56146 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"