The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/50 - 1/100. Predicted molecular weight: 48 kDa.
Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
Involvement in disease
Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
Belongs to the adenosylhomocysteinase family.
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Flow cytometric analysis of K562 cells labeling S adenosylhomocysteine hydrolase with ab170285 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human uterus tissue labeling S adenosylhomocysteine hydrolase with ab170285 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot - Anti-S adenosylhomocysteine hydrolase antibody (ab170285)
Anti-S adenosylhomocysteine hydrolase antibody - N-terminal (ab170285) at 1/50 dilution + Mouse liver tissue lysate at 35 µg
Secondary Anti-rabbit HRP conjugated antibody at 1/10000 dilution