Anti-S adenosylhomocysteine hydrolase antibody - N-terminal (ab170285)

Overview

  • Product name
    Anti-S adenosylhomocysteine hydrolase antibody - N-terminal
    See all S adenosylhomocysteine hydrolase primary antibodies
  • Description
    Rabbit polyclonal to S adenosylhomocysteine hydrolase - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Cynomolgus monkey
  • Immunogen

    Synthetic peptide within Human S adenosylhomocysteine hydrolase aa 86-118 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P23526

  • Positive control
    • Mouse liver tissue lysate; Human uterus tissue; K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/50 - 1/100. Predicted molecular weight: 48 kDa.

Target

  • Function
    Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
  • Pathway
    Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
  • Involvement in disease
    Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
  • Sequence similarities
    Belongs to the adenosylhomocysteinase family.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosylhomocysteinase antibody
    • AdoHcyase antibody
    • ahcY antibody
    • S adenosyl L homocysteine hydrolase antibody
    • S adenosylhomocysteine hydrolase antibody
    • S-adenosyl-L-homocysteine hydrolase antibody
    • SAHH antibody
    • SAHH_HUMAN antibody
    see all

Images

  • Flow cytometric analysis of K562 cells labeling S adenosylhomocysteine hydrolase with ab170285 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human uterus tissue labeling S adenosylhomocysteine hydrolase with ab170285 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Anti-S adenosylhomocysteine hydrolase antibody - N-terminal (ab170285) at 1/50 dilution + Mouse liver tissue lysate at 35 µg

    Secondary
    Anti-rabbit HRP conjugated antibody at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 48 kDa

References

ab170285 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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