Overview

  • Product nameAnti-S tag antibody [SBSTAGa]
    See all S tag primary antibodies
  • Description
    Mouse monoclonal [SBSTAGa] to S tag
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, Flow Cyt, ELISA, Purificationmore details
  • Immunogen

    Recombinant full length protein (see pET-32a-c(+) vector from Novagen)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberSBSTAGa
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/100.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
Purification Use at an assay dependent concentration.

Target

  • RelevanceS tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A.
  • Alternative names
    • KETAAAKFERQHMDS tag antibody antibody
    • S peptide antibody
    • S peptide epitope tag antibody

Anti-S tag antibody [SBSTAGa] images

  • ab24838 staining human HeLa cells by ICC/IF.  Cells were PFA fixed and blocked with 3% BSA for 1 hour at 25°C.  The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C.  A Cy2® conjugated donkey anti-mouse antibody, diluted 1/300 was used as the secondary. 

    The slide was stained with Dapi, S Tag-Cy2 and CREST-Cy3.

    See Abreview

References for Anti-S tag antibody [SBSTAGa] (ab24838)

This product has been referenced in:
  • Makhmoudova A  et al. Identification of multiple phosphorylation sites on maize endosperm starch branching enzyme IIb, a key enzyme in amylopectin biosynthesis. J Biol Chem 289:9233-46 (2014). WB . Read more (PubMed: 24550386) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Gel Running Conditions Reduced Denaturing (7.5%)
Loading amount 15 µg
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 19 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3.0% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 18 2008

Thank you for your enquiry. Yes, ab24838 and ab24839 can be used together in a sandwich ELISA as they recognize different regions of the peptide epitope tag. We unfortunately do not have information regarding the antibodies' association and dissociatio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"