Recombinant Anti-S100 alpha 2/S100A2 antibody [EPR5392] (ab109494)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5392] to S100 alpha 2/S100A2
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-S100 alpha 2/S100A2 antibody [EPR5392]
See all S100 alpha 2/S100A2 primary antibodies -
Description
Rabbit monoclonal [EPR5392] to S100 alpha 2/S100A2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HACAT, BxPC3, HeLa and A431 cell lysates; IHC: Human skin and human lung carcinoma tissues IP: HaCaT. ICC/IF: A431 (Human epidermoid carcinoma epithelial cell) cells Flow cyto(intra): A431 (Human epidermoid carcinoma epithelial cell) cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5392 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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Function
May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth. -
Tissue specificity
A subset of epithelial cells including normal human mammary epithelial cells and keratinocytes. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Developmental stage
Preferentially expressed in normal human mammary epithelial cells as opposed to tumor-derived ones. The level of S100L was shown to correlate inversely with tumor progression. - Information by UniProt
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Database links
- Entrez Gene: 6273 Human
- Omim: 176993 Human
- SwissProt: P29034 Human
- Unigene: 516484 Human
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Alternative names
- CAN19 antibody
- MGC111539 antibody
- Protein S 100L antibody
see all
Images
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Intracellular Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling S100 alpha 2 with purified ab109494 at 1/20 dilution (10 ug/ml) (Red). Cells were fixed with 80% Methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling S100 alpha 2 using ab109494. The cells were fixed with 100% Methanol then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab109494 at 1:100 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody. -
Immunohistochemical analysis of Paraffin-embedded sections human skin tissue labelling S100 alpha 2 with ab109494 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human skin tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemical analysis of Paraffin-embedded sections human lung carcinoma tissue labelling S100 alpha 2 with ab109494 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human lung carcinoma tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-S100 alpha 2/S100A2 antibody [EPR5392] (ab109494) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : HaCaT (Human skin keratinocyte) whole cell lysate at 15 µg
Lane 3 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 4 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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S100 alpha 2/S100A2 was immunoprecipitated from 0.35 mg HaCaT (Human skin keratinocyte) whole cell lysate 10 µg with 109494 at 1/20 dilution (1µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HaCaT (Human skin keratinocyte) whole cell lysate 10 µg
Lane 2: ab109494 IP in HaCaT whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109494 in HaCaT whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab109494 has been referenced in 12 publications.
- Yin A et al. Integrative analysis identifies an immune-relevant epigenetic signature for prognostication of non-G-CIMP glioblastomas. Oncoimmunology 10:1902071 (2021). PubMed: 33854822
- Liu C et al. Development and external validation of a composite immune-clinical prognostic model associated with EGFR mutation in East-Asian patients with lung adenocarcinoma. Ther Adv Med Oncol 13:17588359211006949 (2021). PubMed: 33889215
- Wang H et al. miR-325-3p Promotes the Proliferation, Invasion, and EMT of Breast Cancer Cells by Directly Targeting S100A2. Oncol Res 28:731-744 (2021). PubMed: 33419488
- Chen Y et al. S100A2 Is a Prognostic Biomarker Involved in Immune Infiltration and Predict Immunotherapy Response in Pancreatic Cancer. Front Immunol 12:758004 (2021). PubMed: 34887861
- Raghavan S et al. Microenvironment drives cell state, plasticity, and drug response in pancreatic cancer. Cell 184:6119-6137.e26 (2021). PubMed: 34890551