Anti-S100 alpha 2 antibody [EPR5392] (ab109494)


  • Product name
    Anti-S100 alpha 2 antibody [EPR5392]
    See all S100 alpha 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5392] to S100 alpha 2
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues at the C-terminus of Human S100 alpha 2.

  • Positive control
    • HACAT, BxPC3, and A431 cell lysates; Human skin and Human squamous cell cervical carcinoma tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-S100 alpha 2 antibody (Alexa Fluor® 488) [EPR5392] (ab205456)

    Anti-S100 alpha 2 antibody [EPR5392] (HRP) (ab205754)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab109494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Function
    May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth.
  • Tissue specificity
    A subset of epithelial cells including normal human mammary epithelial cells and keratinocytes.
  • Sequence similarities
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Developmental stage
    Preferentially expressed in normal human mammary epithelial cells as opposed to tumor-derived ones. The level of S100L was shown to correlate inversely with tumor progression.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAN19 antibody
    • MGC111539 antibody
    • Protein S 100L antibody
    • Protein S-100L antibody
    • Protein S100 A2 antibody
    • Protein S100-A2 antibody
    • Protein S100L antibody
    • S100 A2 antibody
    • S100 calcium binding protein A2 antibody
    • S100 calcium-binding protein A2 antibody
    • S100A2 antibody
    • S100L antibody
    • S10A2_HUMAN antibody
    see all

Anti-S100 alpha 2 antibody [EPR5392] images

  • Overlay histogram showing A431 cells stained with ab109494 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109494, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-S100 alpha 2 antibody [EPR5392] (ab109494) at 1/1000 dilution

    Lane 1 : HACAT cell lysate
    Lane 2 : BxPC3 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 11 kDa
  • ab109494 at 1/250 dilution staining S100 alpha 2 in paraffin-embedded Human squamous cell cervical carcinoma tissue
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using ab109494 at 1/250 dilution.

References for Anti-S100 alpha 2 antibody [EPR5392] (ab109494)

This product has been referenced in:
  • Kumar M  et al. Prognostic significance of cytoplasmic S100A2 overexpression in oral cancer patients. J Transl Med 13:8 (2015). IHC-P ; Human . Read more (PubMed: 25591983) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab109494.
Please use the links above to contact us or submit feedback about this product.


Sign up