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Rabbit polyclonal to S100 alpha 6
Recombinant fragment corresponding to a region within amino acids 1-90 of Human S100 alpha 6 (Uniprot ID: P06703).
U-87 MG whole cell lysate; A431 cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
1/100 - 1/1000.
May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
Belongs to the S-100 family.
Contains 2 EF-hand domains.
The N-terminus is blocked.
Nucleus envelope. Cytoplasm. Cell membrane.
Information by UniProt
Western blot - Anti-S100 alpha 6 antibody (ab154955)
Anti-S100 alpha 6 antibody (ab154955) at 1/5000 dilution + U-87 MG whole cell lysate at 30 µg
Predicted band size: 10 kDa 15% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6 antibody (ab154955)
Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labeling S100 alpha 6 with ab154955 at 1/500 dilution (green). Lower image shows cells co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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