Overview

  • Product nameAnti-S100 alpha 6 antibody
    See all S100 alpha 6 primary antibodies
  • Description
    Mouse monoclonal to S100 alpha 6
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein: MACPLDQAIG LLVAIFHKYS GREGDKHTLS KKELKELIQK ELTIGSKLQD AEIARLMEDL DRNKDQEVNF QEYVTFLGAL ALIYNEALKG , corresponding to amino acids 1-91 of Human S100 alpha 6

Properties

Applications

Our Abpromise guarantee covers the use of ab55680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 10 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationNucleus envelope. Cytoplasm. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2A9 antibody
    • 5B10 antibody
    • CABP antibody
    • CACY antibody
    • Calcyclin antibody
    • Growth factor inducible protein 2A9 antibody
    • Growth factor-inducible protein 2A9 antibody
    • MLN 4 antibody
    • MLN4 antibody
    • OTTHUMP00000015472 antibody
    • OTTHUMP00000015473 antibody
    • PRA antibody
    • PRAGrowth factor inducible protein 2A9 antibody
    • Prolactin receptor associated protein antibody
    • Prolactin receptor-associated protein antibody
    • Protein S100 A6 antibody
    • Protein S100-A6 antibody
    • S100 A6 antibody
    • S100 calcium binding protein A6 (calcyclin) antibody
    • S100 calcium binding protein A6 antibody
    • S100 calcium-binding protein A6 antibody
    • S100A6 antibody
    • S10A6_HUMAN antibody
    see all

Anti-S100 alpha 6 antibody images

  • Overlay histogram showing A549 cells stained with ab55680 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55680, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • S100 alpha 6 antibody (ab55680) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach carcinoma.


  • Predicted band size : 10 kDa
    S100 alpha 6 antibody (ab55680) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References for Anti-S100 alpha 6 antibody (ab55680)

This product has been referenced in:
  • Ralhan R  et al. Immunohistochemical Subcellular Localization of Protein Biomarkers Distinguishes Benign from Malignant Thyroid Nodules: Potential for Fine-Needle Aspiration Biopsy Clinical Application. Thyroid 25:1224-34 (2015). IHC . Read more (PubMed: 26131603) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (urine)
Loading amount 0.6 µg
Specification urine
Gel Running Conditions Reduced Denaturing (18% gel)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (metastatic melanoma)
Specification metastatic melanoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, ER2
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Dec 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"