The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 10 kDa.
Use a concentration of 3 µg/ml.
Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionMay function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
Sequence similaritiesBelongs to the S-100 family. Contains 2 EF-hand domains.
Post-translational modificationsThe N-terminus is blocked.
Overlay histogram showing A549 cells stained with ab55680 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55680, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
IHC-P - S100 alpha 6 antibody (ab55680)
S100 alpha 6 antibody (ab55680) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach carcinoma.
Western blot - S100 alpha 6 antibody (ab55680)
Predicted band size : 10 kDa S100 alpha 6 antibody (ab55680) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-S100 alpha 6 antibody (ab55680)
This product has been referenced in:
Ralhan R et al. Immunohistochemical Subcellular Localization of Protein Biomarkers Distinguishes Benign from Malignant Thyroid Nodules: Potential for Fine-Needle Aspiration Biopsy Clinical Application. Thyroid25:1224-34 (2015).
Read more (PubMed: 26131603) »