Anti-S100 alpha antibody [2C8B8] (ab52271)
Key features and details
- Mouse monoclonal [2C8B8] to S100 alpha
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-S100 alpha antibody [2C8B8]
See all S100 alpha primary antibodies -
Description
Mouse monoclonal [2C8B8] to S100 alpha -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Ni-NTA purified truncated recombinant S100A expressed in E. Coli
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Positive control
- Human brain, human lymphoid follicle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2C8B8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
Tissue specificity
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6271 Human
- Omim: 176940 Human
- SwissProt: P23297 Human
- Unigene: 515715 Human
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Alternative names
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab52271 has not yet been referenced specifically in any publications.