Anti-S100 alpha antibody [EPR5251] (ab109384)

Overview

  • Product nameAnti-S100 alpha antibody [EPR5251]
    See all S100 alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR5251] to S100 alpha
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human S100 alpha

  • Positive control
    • WB: kidney, C2C12, mouse kidney, Human brain, HT-1080 and mouse heart lysate IF: HeLa cells
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
    • Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
    • Sequence similaritiesBelongs to the S-100 family.
      Contains 2 EF-hand domains.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Protein S100-A1 antibody
      • S-100 protein alpha chain antibody
      • S-100 protein subunit alpha antibody
      • S100 antibody
      • S100 Calcium Binding Protein A1 antibody
      • S100 calcium-binding protein A1 antibody
      • S100 protein alpha polypeptide antibody
      • S100A antibody
      • s100a1 antibody
      • S10A1_HUMAN antibody
      see all

    Anti-S100 alpha antibody [EPR5251] images

    • All lanes : Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution

      Lane 1 : Rat kidney lysate
      Lane 2 : C2C12 lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Human brain lysate
      Lane 5 : HT-1080 lysate
      Lane 6 : Mouse heart lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 11 kDa
    • ab109384, at a 1/100 dilution staining S100 alpha in HeLa cells
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-S100 alpha antibody [EPR5251] (ab109384)

    ab109384 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"