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Product name Anti-S100 alpha antibody [EPR5251] See all S100 alpha primary antibodies
Rabbit monoclonal [EPR5251] to S100 alpha
Tested applications Suitable for:
WB, IP, Flow Cyt, ICC/IF more details Unsuitable for:
Mouse, Rat, Human
A synthetic peptide corresponding to residues in Human S100 alpha
WB: kidney, C2C12, mouse kidney, Human brain, HT-1080 and mouse heart lysate
IF: HeLa cells
Produced using Abcam's RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
This product is a recombinant rabbit monoclonal antibody.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K D)
K D = 5.00 x 10
Learn more about K D
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR5251
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
1/10 - 1/100.
1/10 - 1/100.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
Application notes Is unsuitable for IHC-P.
Function Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Tissue specificity Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Sequence similarities Belongs to the S-100 family. Contains 2 EF-hand domains.
Cellular localization Cytoplasm.
Information by UniProt
Protein S100-A1 antibody
S-100 protein alpha chain antibody
S-100 protein subunit alpha antibody
Anti-S100 alpha antibody [EPR5251] images
Western blot - S100 alpha antibody [EPR5251] (ab109384)
All lanes : Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution Lane 1 : Rat kidney lysate Lane 2 : C2C12 lysate Lane 3 : Mouse kidney lysate Lane 4 : Human brain lysate Lane 5 : HT-1080 lysate Lane 6 : Mouse heart lysate Lysates/proteins at 10 µg per lane. Predicted band size : 11 kDa
Immunocytochemistry/ Immunofluorescence - S100 alpha antibody [EPR5251] (ab109384)
ab109384, at a 1/100 dilution staining S100 alpha in HeLa cells
Other-Anti-S100 alpha antibody [EPR5251](ab109384)
References for Anti-S100 alpha antibody [EPR5251] (ab109384)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"