Overview

  • Product nameAnti-S100 antibody [15E2E2]
    See all S100 primary antibodies
  • Description
    Mouse monoclonal [15E2E2] to S100
  • Tested applicationsSuitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    S100 Protein A and B subunits

  • Positive control
    • Human Melanoma or Schwannoma lymphoblastic leukemia tissue. ab86728 stains melanocytes, schwannomas, peripheral neural tissue and astrocytes, and benign and malignant melanomas and their metastases.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
Flow Cyt 1/1000. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Anti-S100 antibody [15E2E2] images

  • Overlay histogram showing SHSY-5Y cells stained with ab86728 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86728, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-S100 antibody [15E2E2] (ab86728)

ab86728 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"