For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Full length native protein (purified) (Human brain).
Our Abpromise guarantee covers the use of ab14849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.|
|IHC-P||1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See Abreview.|
ab14849 staining S100 in human melanoma cultured cells by ICC (Immunocytochemistry). Cells were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.2% Triton X-100 for 15 minutes and blocked for 30 minutes at 21°C. Samples were incubated with primary antibody (at concentrations listed above) for 15 hours at 4°C. A HRP-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.
ab14849 staining S100 in the human Melanoma cell line 1205Lu xenograph tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.1% Triton X100 and blocked with a commercial block solution for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris EDTA at pH 9. The sample was incubated with primary antibody (1/300 in blocking solution) at 4°C for 18 hours. A biotin-conjugated goat polyclonal to mouse IgG (1/50) was used as secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"