Overview

  • Product name
    Anti-S100 antibody [4B3]
    See all S100 primary antibodies
  • Description
    Mouse monoclonal [4B3] to S100
  • Host species
    Mouse
  • Specificity
    Specific for alpha-beta heterodimer and beta-beta homodimer.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length native protein (purified) (Human brain).

  • Positive control
    • ICC: Human melanoma cultured cells. WB: Purified S100 protein from human brain. IHC-P: Rat brain tissue. Human melanoma cell line 1205Lu xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab14849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
IHC-P 1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See Abreview.
IHC-Fr 1/100.
ICC 1/500.

Target

  • Function
    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificity
    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similarities
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Images

  • ab14849 at 1/3000 staining rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the antibody. A biotinylated goat anti-mouse IgG was used as the secondary.

    See Abreview

  • ab14849 staining S100 in human melanoma cultured cells by ICC (Immunocytochemistry). Cells were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.2% Triton X-100 for 15 minutes and blocked for 30 minutes at 21°C. Samples were incubated with primary antibody (at concentrations listed above) for 15 hours at 4°C. A HRP-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Interaction of several monoclonal antibodies with purified S100 protein (1 µg) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system.

    Lane 1 = ab14849, Lane 2 = ab8330, ab Lane 3 = ab10203, Lane 4 = ab8334.

    The doublet band appears at about 20-21 kD.

  • ab14849 staining S100 in the human Melanoma cell line 1205Lu xenograph tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde, permeabilized with 0.1% Triton X100 and blocked with a commercial block solution for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris EDTA at pH 9. The sample was incubated with primary antibody (1/300 in blocking solution) at 4°C for 18 hours. A biotin-conjugated goat polyclonal to mouse IgG (1/50) was used as secondary antibody.

    See Abreview

References

This product has been referenced in:

See all 9 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Blocking step
from Novolink Kit as blocking agent for 30 minute(s) · Concentration: 0.4µg/mL · Temperature: 21°C
Sample
Human Cultured Cells (Melanoma)
Specification
Melanoma
Permeabilization
Yes - 0.2% Triton-X, 15 min
Fixative
Paraformaldehyde
Username

Stacey Dmello

Verified customer

Submitted Jul 23 2013

Thank you for contacting us and your patience.

We recommend to use purified antibody in a concentration og 1ug/ml for WB. This recommended concentration needs to be optimised for each sample and experiment by the user but it is a good approxim...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (C57B/J mouse Vagus Nerve)
Specification
C57B/J mouse Vagus Nerve
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Mar 06 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Marmoset colon)
Specification
Marmoset colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21C°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Melanoma cell line 1205Lu xenopraph)
Specification
Melanoma cell line 1205Lu xenopraph
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH9.0
Permeabilization
Yes - 0.1% triton x-100
Blocking step
Commercially available block solution as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Hongwei Shao

Verified customer

Submitted Mar 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (rat brain)
Specification
rat brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: No buffer: citric acid pH6
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jul 16 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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