Anti-S100 antibody [6G1] (HRP) (ab10203)

Overview

  • Product name
    Anti-S100 antibody [6G1] (HRP)
    See all S100 primary antibodies
  • Description
    Mouse monoclonal [6G1] to S100 (HRP)
  • Conjugation
    HRP
  • Specificity
    Clone 6G1 is specific for S-100 beta beta and and S100 alpha beta
  • Tested applications
    Suitable for: ELISA, Sandwich ELISA, Purification, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human S-100 protein

  • General notes
    Concentration varies from lot to lot and can be provided on request.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab10203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [8B10] to S100 (ab8330). Use as Detection antibody. Sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5mM CaCl2 in thses assay buffers. Can be used as detection antibody in conjunction with ab8330 and ab8334.
Purification Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Target

  • Function
    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificity
    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similarities
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Images

  • S-100 calibration curve, from one step assay in streptavidin coated plates. Ab8330 [200 ng/well] was used as capture and Eu- labelled ab10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20°C.

References

ab10203 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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