Anti-S100 antibody [6G1] (HRP) (ab10203)

Overview

  • Product nameAnti-S100 antibody [6G1] (HRP)
    See all S100 primary antibodies
  • Description
    Mouse monoclonal [6G1] to S100 (HRP)
  • ConjugationHRP
  • SpecificityClone 6G1 is specific for S-100 beta beta and and S100 alpha beta
  • Tested applicationsSuitable for: ELISA, Sandwich ELISA, Purification, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human S-100 protein

  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab10203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [8B10] to S100 (ab8330) and Mouse monoclonal [3B10] to S100 (ab8334). Use as Detection antibody. Sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5mM CaCl2 in thses assay buffers. Can be used as detection antibody in conjunction with ab8330 and ab8334.
Purification Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Target

  • FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Anti-S100 antibody [6G1] (HRP) images

  • S-100 calibration curve, from one step assay in streptavidin coated plates. Ab8330 [200 ng/well] was used as capture and Eu- labelled ab10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20°C.

References for Anti-S100 antibody [6G1] (HRP) (ab10203)

ab10203 has not yet been referenced specifically in any publications.

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