Overview

  • Product nameAnti-S100 antibody [8B10]See all S100 primary antibodies ...
  • Description
    Mouse monoclonal [8B10] to S100
  • SpecificitySpecific to S-100 beta beta and alpha beta. It does not recognize the alpha-alpha form or alpha subunit only.
  • Tested applicationsELISA, IHC - Wholemount, ICC/IF, WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length protein (Human).

  • Positive control
    • Human brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab8330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IHC - Wholemount Use at an assay dependent concentration. PubMed: 21856924
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [6G1] to S100 (HRP) (ab10203). Sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5mM CaCL2 in these assay buffers. Can be used as capture antibody in conjunction with ab10203.

Target

  • FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S 100 protein alpha chain antibody
    • S 100 PROTEIN BETA CHAIN antibody
    • S 100 protein beta subunit antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium binding protein beta neural antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha chain antibody
    • S100 protein alpha polypeptide antibody
    • S100 protein beta polypeptide neural antibody
    • S100A antibody
    • S100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Anti-S100 antibody [8B10] images

  • S-100 calibration curve, from one step assay in streptavidin coated plates. Ab8330 [200 ng/well] was used as capture and Eu- labelled ab10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20°C.
  • ab8330 at a dilution of 1/1000, staining S100 (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 20x objective and [B] 40x objective.

    NB: No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
     

References for Anti-S100 antibody [8B10] (ab8330)

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (Embryonic and postnatal mouse cerebellum)
Specification Embryonic and postnatal mouse cerebellum
Permeabilization Yes - 0.5% Triton in PBS
Fixative Paraformaldehyde
Username

Qiuxia Guo

Verified customer

Submitted Nov 01 2013

Thank you for contacting us and sorry for the delay in getting back to you.

Kate is unfortunately still not in the office but I will try to answer your questions as best I can.

I can confirm that the antibodies mentioned (ab8330 and a...

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Thank you for your reply.

My sincere apologies for the confusion. In this case, I am sorry we do not have a suitable standard protein to suggest on this occasion.

In answer to your second question regarding ab48653 and ab105371, I...

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Thank you for your follow up message, and I appreciate your patience waiting for a response. I have been collecting information from the originator of these antibodies.

Reviewing the details for our S100 antibodies, I can confirm the followi...

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Thank you for your inquiry.

I am happy to confirm that we do have an antibody in our catalogue that can be used as astrocyte marker and is tested and guaranteed for IHC- whole mounts:

ab8330

http://www.abcam.com/index.html...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring...

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I have contacted the lab regarding the epitope for ab8330 and received the following answer. "Unfortunately we have not determined the epitope for anti-S100 ab8330. The only information we have is that according to our internal tests ab8330 recogn...

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Thank you for your enquiry and also your patience. I would actually recommend that you pair ab8330 and ab10203 or ab8334 and ab10203. I will update the datasheets to reflect this also. I have also included our protocol for use with these antibod...

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Thank you for your reply. We would like to confirm that ab8330 recognizes both the alpha-beta and beta-beta forms of S-100 in Western blotting. A western blotting image with the legend can be seen on our on-line product datasheet. Western blott...

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Thank you for your enquiry and your interest in our products. We agree with you, it is always very important to use positive control. Human brain lysate itself or lysate from astrocytes, Schwann's cells, ependymomas and astrogliomas can be used as ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"