Full length native S100 protein (purified) (Bovine)
Our Abpromise guarantee covers the use of ab76729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/50 - 1/100.|
|IHC-Fr||1/50 - 1/100.|
ab76729 staining S100 in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with 5% BSA for 1 hour at 250C. The sample was incubated with primary antibody (1/100) for 12 hours at 40C in PBS. A Cy3®-conjugated donkey polyclonal to rabbit IgG was used as secondary antibody at 1/500 dilution.