Overview

  • Product nameAnti-S100 antibody - Astrocyte Marker
    See all S100 primary antibodies
  • Description
    Rabbit polyclonal to S100 - Astrocyte Marker
  • SpecificityThis antibody recognizes all the S-100 isoforms, proteins of 21-24kDa identified as the A and B subunits of S100 protein. It stains schwannomas, ependymomas, astrogliomas, and almost all benign and malignant melanomas and their metastases. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
  • Tested applicationsSuitable for: IHC (PFA fixed), IHC-FoFr, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    Recombinant full length protein (Cow).

  • Positive control
    • Melanoma or Schwannoma
  • General notesContains a proprietary preservative that is not sodium azide or thimerosal

Properties

Applications

Our Abpromise guarantee covers the use of ab868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) Use at an assay dependent concentration. PubMed: 25184527
IHC-FoFr Use at an assay dependent concentration. PubMed: 20633557
IHC-P 1/100 - 1/200.
WB Use at an assay dependent concentration. PubMed: 18032704
ICC/IF Use at an assay dependent concentration. PubMed: 19593677

Target

  • FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Anti-S100 antibody - Astrocyte Marker images

  • ab868 staining mouse brain tissue sections by IHC-P.  Sections were formaldehyde fixed and permeabilized in 0.1% Triton-X100 in blocking solution prior to blocking in 100% BioGenex Power Block Universal Blocking Reagent for 10 minutes at 22°C.  The primary antibody was diluted 1/1000 in 1% BSA in PBS pH 7.4 and incubated with the section for 1 hour at 22°C.  A HRP polymer conjugated anti-rabbit antibody was used as the secondary.   A tyramide Alexa Fluor® 594 conjugate was added and the signal was visualized using a fluorescent microscope and a rhodamine filter

    See Abreview

  • ab868 staining S100 in mouse cerebellum by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were paraformaldehyde fixed prior to blocking in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/400, was used as the secondary antibody.

    See Abreview

  • ab868 staining S100 - Astrocyte Marker in Mouse hippocampus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. An undiluted HRP-conjugated polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Melanoma section labelling S100 with ab868 at 1/100 - 1/200 dilution. The tissue was counterstained with hematoxylin.

References for Anti-S100 antibody - Astrocyte Marker (ab868)

This product has been referenced in:
  • Krencik R  et al. Dysregulation of astrocyte extracellular signaling in Costello syndrome. Sci Transl Med 7:286ra66 (2015). ICC . Read more (PubMed: 25947161) »
  • Torigoe M  et al. Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium. Sci Rep 5:12258 (2015). IHC-FoFr ; Mouse . Read more (PubMed: 26193445) »

See all 16 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1080427.

To check the status of the order please con...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Penguin Tissue sections (brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
Permeabilization No
Specification brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
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Verified customer

Submitted Apr 12 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (nerve)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization No
Specification nerve
Blocking step Vector Lab's M.O.M kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Jun 04 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Trigeminal Ganglia)
Antigen retrieval step None
Permeabilization No
Specification Trigeminal Ganglia
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
Username

April Rempel

Verified customer

Submitted Apr 30 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH 6.0
Sample Rat Tissue sections (nerve)
Specification nerve
Permeabilization No
Fixative Formaldehyde
Username

Dr. Lynn Dong

Verified customer

Submitted Aug 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step None
Sample Rat Tissue sections (rat nerve)
Specification rat nerve
Permeabilization Yes - 0.2% Triton X100
Fixative Formaldehyde
Username

Dr. Lynn Dong

Verified customer

Submitted Aug 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Enzymatic
Sample Mouse Tissue sections (Skin)
Specification Skin
Permeabilization No
Fixative Paraformaldehyde
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Submitted Jul 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cultured Cells (differentiating Neural stem cells from Dentate Gyr)
Specification differentiating Neural stem cells from Dentate Gyr
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton-X-100 for 30 min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Nov 01 2012

Thank you for contacting us. Although S100 is an known astrocyte marker, I do not think that S100 recognizes ALL astrocytes. I did find that Aldh1L1 is a marker known to have a much broader range than GFAP (Cahoy JD et al (2008) Journal of Neuroscience...

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Following up my previous message regarding specificity of the S100 antibody ab868, the laboratory has confirmed that the statement regarding detection of 2-24 kDa protein refers to the dimers of the subunits. Recognition of the A8 and A9 isoforms has n...

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