Anti-S100 antibody - Astrocyte Marker (ab868)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 antibody - Astrocyte Marker (ab868)

ab868 staining mouse brain tissue sections by IHC-P.  Sections were formaldehyde fixed and permeabilized in 0.1% Triton-X100 in blocking solution prior to blocking in 100% BioGenex Power Block Universal Blocking Reagent for 10 minutes at 22°C.  The primary antibody was diluted 1/1000 in 1% BSA in PBS pH 7.4 and incubated with the section for 1 hour at 22°C.  A HRP polymer conjugated anti-rabbit antibody was used as the secondary.   A tyramide Alexa Fluor® 594 conjugate was added and the signal was visualized using a fluorescent microscope and a rhodamine filter

This image is courtesy of an anonymous Abreview

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 antibody - Astrocyte Marker (ab868)

ab868 staining S100 in mouse cerebellum by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were paraformaldehyde fixed prior to blocking in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/400, was used as the secondary antibody.

This image is courtesy of an Abreview submitted by P Vig

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 antibody - Astrocyte Marker (ab868)

ab868 staining S100 - Astrocyte Marker in Mouse hippocampus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. An undiluted HRP-conjugated polyclonal was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Dr Markus Kipp

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