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ab79995 |
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Read our guarantee »Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Anti-S100 antibody - Astrocyte Marker
See all S100 products (24) ...
Rabbit polyclonal to S100 - Astrocyte Marker
This antibody recognizes all the S-100 isoforms, proteins of 21-24kDa identified as the A and B subunits of S100 protein. It stains schwannomas, ependymomas, astrogliomas, and almost all benign and malignant melanomas and their metastases. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
IHC (PFA fixed), IHC-FoFr, IHC-P, WB, ICC/IFmore details
Reacts with
Mouse, Rat, Cow, Human, Pig
Recombinant full length protein (Cow).
Melanoma or Schwannoma
Liquid
Store at +4°C.
Preservative: 0.1% Sodium Azide
Constituents: PBS, Carrier protein, Da Vinci Green Diluent, pH 7.3
Whole antiserum
Polyclonal
unknown
IgG
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class IV >> Neurofilaments
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 antibody - Astrocyte Marker (ab868)
(enlarge)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 antibody - Astrocyte Marker (ab868)
(enlarge)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 antibody - Astrocyte Marker (ab868)
(enlarge)
Our Abpromise guarantee covers the use of ab868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (PFA fixed): Use at an assay dependent concentration.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 20633557)
IHC-P: Use at an assay dependent concentration.
WB: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Belongs to the S-100 family.
Contains 2 EF-hand domains.
Cytoplasm.
Target information above from: UniProt accessionP23297
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 antibody - Astrocyte Marker (ab868)

ab868 staining mouse brain tissue sections by IHC-P. Sections were formaldehyde fixed and permeabilized in 0.1% Triton-X100 in blocking solution prior to blocking in 100% BioGenex Power Block Universal Blocking Reagent for 10 minutes at 22°C. The primary antibody was diluted 1/1000 in 1% BSA in PBS pH 7.4 and incubated with the section for 1 hour at 22°C. A HRP polymer conjugated anti-rabbit antibody was used as the secondary. A tyramide Alexa Fluor® 594 conjugate was added and the signal was visualized using a fluorescent microscope and a rhodamine filter
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 antibody - Astrocyte Marker (ab868)

ab868 staining S100 in mouse cerebellum by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were paraformaldehyde fixed prior to blocking in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/400, was used as the secondary antibody.
This image is courtesy of an Abreview submitted by P Vig
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 antibody - Astrocyte Marker (ab868)

ab868 staining S100 - Astrocyte Marker in Mouse hippocampus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. An undiluted HRP-conjugated polyclonal was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Dr Markus Kipp
This product has been referenced in:
See all 4 publications for this product
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ab868 staining mouse brain tissue sections by IHC-P. Sections were formaldehyde fixed and permeabilized in 0.1% Triton-X100 in blocking solution prior to blocking in 100% BioGenex Power Block Universal Blocking Reagent for 10 minutes at 22°C. The primary antibody was diluted 1/1000 in 1% BSA in PBS pH 7.4 and incubated with the section for 1 hour at 22°C. A HRP polymer conjugated anti-rabbit antibody was used as the secondary. A tyramide Alexa Fluor® 594 conjugate was added and the signal was visualized using a fluorescent microscope and a rhodamine filter
This image is courtesy of an anonymous Abreview

ab868 staining S100 in mouse cerebellum by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were paraformaldehyde fixed prior to blocking in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/400, was used as the secondary antibody.
This image is courtesy of an Abreview submitted by P Vig

ab868 staining S100 - Astrocyte Marker in Mouse hippocampus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. An undiluted HRP-conjugated polyclonal was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Dr Markus Kipp
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