|
|
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 antibody - Astrocyte Marker (ab868)
(enlarge)
|
|
|
|
|
Product Name
|
S100 antibody - Astrocyte Marker
|
| |
See all S100 antibodies (23)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to S100 - Astrocyte Marker
|
|
Immunogen
|
Recombinant full length protein (Cow).
|
|
Reacts with
(species key)
|
Hu, Ms, Rat, Cow, Pig
|
|
Specificity
|
This antibody recognizes all the S-100 isoforms, proteins of 21-24kDa identified as the A and B subunits of S100 protein. It stains schwannomas, ependymomas, astrogliomas, and almost all benign and malignant melanomas and their metastases. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
|
|
Tested applications
(see key)
|
ICC/IF, IHC (PFA fixed), IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent concentration.
IHC (PFA fixed): Use at an assay dependent concentration.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Melanoma or Schwannoma
|
|
Cellular localization
|
Cytoplasmic and Nuclear
|
|
Research areas
|
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class IV >> Neurofilaments
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
|
|
Relevance
|
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein may function in stimulation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. Immunoreactive S100 protein localizes in the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. Ganglion cells do not stain for S100 protein. S100 can be detected in almost all benign naevi and malignant melanocytic tumours of the skin, and in Langerhans cells in the skin.
|
|
|
S100 antibody - Astrocyte Marker (ab868) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Whole antiserum
|
|
Storage buffer
|
Preservative: 0.1% Sodium Azide
Constituents: PBS, Carrier protein, Da Vinci Green Diluent, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Concentration not determined - why is this?
|
|
Storage instructions
|
Store at +4°C.
|
|
Notes
|
Contains a proprietary preservative that is not sodium azide or thimerosal
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this S100 antibody - Astrocyte Marker is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for S100 antibody - Astrocyte Marker images, references, products related to ab868 and other tools.
|
| |
S100 antibody - Astrocyte Marker images:
|
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 antibody - Astrocyte Marker (ab868)
ab868 staining mouse brain tissue sections by IHC-P. Sections were formaldehyde fixed and permeabilized in 0.1% Triton-X100 in blocking solution prior to blocking in 100% BioGenex Power Block Universal Blocking Reagent for 10 minutes at 22°C. The primary antibody was diluted 1/1000 in 1% BSA in PBS pH 7.4 and incubated with the section for 1 hour at 22°C. A HRP polymer conjugated anti-rabbit antibody was used as the secondary. A tyramide Alexa Fluor® 594 conjugate was added and the signal was visualized using a fluorescent microscope and a rhodamine filter This image is courtesy of an anonymous Abreview
See Abreview
|
| |
References for S100 antibody - Astrocyte Marker (ab868)
|
|
This product has been used in: (two most recent references)
Vig PJ et al. Bergmann glial S100B activates myo-inositol monophosphatase 1 and Co-localizes to purkinje cell vacuoles in SCA1 transgenic mice. Cerebellum 8:231-44 (2009). ICC/IF, IHC-P; Mouse. PubMed: 19593677
Nelson AD et al. A high concentration of epidermal growth factor increases the growth and survival of neurogenic radial glial cells within human neurosphere cultures. Stem Cells 26:348-55 (2008). WB; Human. PubMed: 18032704
See all 5 publication references for this product.
If you publish research using ab868 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to S100
|
| |
S100 antibody - Astrocyte Marker - more information
|
Customer reviews (feedback) regarding S100 antibody - Astrocyte Marker |
Customer FAQs regarding S100 antibody - Astrocyte Marker |
Protocols for S100 antibody - Astrocyte Marker |
Price and availability of products related to S100 antibody - Astrocyte Marker |
| |
S100 antibody - Astrocyte Marker - related products:
|
|
|
| |
S100 antibody - Astrocyte Marker - other tools:
|
Contact Abcam with a Technical Enquiry about S100 antibody - Astrocyte Marker |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
