Recombinant full length protein (Cow).
Our Abpromise guarantee covers the use of ab868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC (PFA fixed)||Use at an assay dependent concentration. PubMed: 25184527|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20633557|
|IHC-P||1/100 - 1/200.|
|WB||Use at an assay dependent concentration. PubMed: 18032704|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19593677|
ab868 staining mouse brain tissue sections by IHC-P. Sections were formaldehyde fixed and permeabilized in 0.1% Triton-X100 in blocking solution prior to blocking in 100% BioGenex Power Block Universal Blocking Reagent for 10 minutes at 22°C. The primary antibody was diluted 1/1000 in 1% BSA in PBS pH 7.4 and incubated with the section for 1 hour at 22°C. A HRP polymer conjugated anti-rabbit antibody was used as the secondary. A tyramide Alexa Fluor® 594 conjugate was added and the signal was visualized using a fluorescent microscope and a rhodamine filter
Immunohistochemical analysis of formalin-fixed paraffin-embedded Melanoma section labelling S100 with ab868 at 1/100 - 1/200 dilution. The tissue was counterstained with hematoxylin.