Overview

  • Product name
    Anti-S100 antibody [B32.1]
    See all S100 primary antibodies
  • Description
    Mouse monoclonal [B32.1] to S100
  • Specificity
    This antibody reacts with alpha and beta subunits of human S-100 protein (21-24 kD).
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Full length native protein (purified) (Cow).

  • Positive control
    • Melanoma tissues containing nerves, such as intestine and pancreas. Brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab7852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000. PubMed: 19365568
IHC-P Use at an assay dependent concentration.
IHC-FoFr 1/500 - 1/1000. 1/500 - 1/1000. Fix with 4% w/v paraformaldehyde and 15% saturated picric acid in 0.1 M. phosphate buffer pH 7.4.

Target

  • Function
    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificity
    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similarities
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Anti-S100 antibody [B32.1] images

  • ab7852 at a dilution of 1/1000, staining Astrocytes (purple; Alexa 488 secondary at 1/2000) on 30µm coronal brain section tissue in free floating IHC (see protocol link for detailed description). [A] Image taken with 20x objective and [B] Image taken with 40x objective. Images coloured in Photoshop.

    NB: No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

References for Anti-S100 antibody [B32.1] (ab7852)

This product has been referenced in:
  • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Monkey . Read more (PubMed: 28190929) »
  • Stokum JA  et al. Heterogeneity of aquaporin-4 localization and expression after focal cerebral ischemia underlies differences in white versus grey matter swelling. Acta Neuropathol Commun 3:61 (2015). IHC-P ; Human . Read more (PubMed: 26419740) »

See all 10 Publications for this product

Product Wall

Thank you for your inquiry. I can confirm that antibody ab7852 should recognize the protein ab54050. But ab7852 (Anti-S100 antibody [B32.1] - Astrocyte Marker) is not tested and guaranteed for WB and it is not known if it binds to denatured S100 ...

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Thank you for your inquiry. Yes, both ab7852 and ab115968 react with the alpha and beta subunits of S-100 protein. Anti-S100 antibody [B32.1] - Astrocyte Marker (ab7852) is €350.00 for 500ul. http://www.abcam.com/S100-antibody-B32-...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Nerve)
Specification
Nerve
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid
Permeabilization
No
Blocking step
non fat milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 4°C
Username

Dr. 哲也 今村

Verified customer

Submitted Jul 17 2008

Application
Immunocytochemistry
Sample
Rat Cultured Cells (BMSC (Bone Marrow Stem Cell))
Specification
BMSC (Bone Marrow Stem Cell)
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 25 2008

Thank you for your enquiry and patience. The originator of ab7852 has informed me that the sections were fixed with formalin. Please contact us again if you have any additional questions.

Thank you for your reply. I'm sorry I didn't see that ab7852 was against S100 and not S100 beta, thank you for noticing this. I cannot guarantee indeed that the protein will not be present in cells other than astrocytes of glial cells,the current l...

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Use enzymatic antigen retrieval using the protocol linked on the right hand side of the Abcam homepage. ABC is recommended as a visualisation technique.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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