The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Ready to use.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Belongs to the S-100 family. Contains 2 EF-hand domains.
ab74161 staining S100 in human melanoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note intense cytoplasmic staining of tumor cells.