Overview

  • Product nameAnti-S100 antibody [S100 217] (FITC)
    See all S100 primary antibodies
  • Description
    Mouse monoclonal [S100 217] to S100 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Monkey
  • Immunogen

    Full length native protein (purified) (Cow)

  • General notesThis antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis.

Properties

Applications

Our Abpromise guarantee covers the use of ab76749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.
ICC/IF Use a concentration of 10 - 20 µg/ml.

Incubate for 2 hours in the dark at RT. The incubation can also be left overnight at 4ºC.

Target

  • FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

Anti-S100 antibody [S100 217] (FITC) images

  • Overlay histogram showing SH-SY5Y cells stained with ab76749 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76749, 0.1μg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [MOPC-21] (FITC) (ab18435, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-S100 antibody [S100 217] (FITC) (ab76749)

This product has been referenced in:
  • Zhu X  et al. Schwann cell proliferation and differentiation that is induced by ferulic acid through MEK1/ERK1/2 signalling promotes peripheral nerve remyelination following crush injury in rats. Exp Ther Med 12:1915-1921 (2016). Rat . Read more (PubMed: 27588110) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"