Overview

  • Product nameAnti-S100 beta antibody [EP1576Y]
    See all S100 beta primary antibodies
  • Description
    Rabbit monoclonal [EP1576Y] to S100 beta
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Goat, Human, Zebrafish, Macaque Monkey
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human S100 beta aa 50 to the C-terminus (C terminal).
    Database link: P04271

  • Positive control
    • IHC-P: Human, Mouse and Rat cerebral cortex. Human spiral ganglion tissue, human melanoma tissue. WB: B16F0 and A-375 cell lysates, Mouse spinal cord, Rat brain. ICC/IF: A-375 cell lysates IP: Human fetal brain
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-S100 beta antibody (Alexa Fluor® 488) [EP1576Y] (ab196442)

    Anti-S100 beta antibody (Alexa Fluor® 647) [EP1576Y] (ab196175)

    Anti-S100 beta antibody (HRP) [EP1576Y] (ab195535)

    Anti-S100 beta antibody (Alexa Fluor® 568) [EP1576Y] (ab201837)

Properties

Applications

Our Abpromise guarantee covers the use of ab52642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000 - 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

 

IP 1/50.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Target

  • FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
  • Tissue specificityAlthough predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
  • Sequence similaritiesBelongs to the S-101 family.
    Contains 2 EF-hand domains.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NEF antibody
    • Protein S100 B antibody
    • Protein S100-B antibody
    • S 100 calcium binding protein beta chain antibody
    • S 100 protein beta chain antibody
    • S-100 protein beta chain antibody
    • S-100 protein subunit beta antibody
    • S100 antibody
    • S100 calcium binding protein beta (neural) antibody
    • S100 calcium-binding protein B antibody
    • S100 protein beta chain antibody
    • S100B antibody
    • S100B_HUMAN antibody
    • S100beta antibody
    see all

Anti-S100 beta antibody [EP1576Y] images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 A-375 (Human malignant melanoma cell line) cells labeling S100 beta with purified ab52642 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) ab150077 secondary antibody at 1/500 dilution (green). The nuclear counter stain is DAPI (blue). The negative control is as follows;
    ab52642 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

     

  • Immunohistochemical analysis of paraffin embedded Human cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

  • All lanes : Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/10000 dilution (purified)

    Lane 1 : Mouse spinal cord
    Lane 2 : Rat brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin embedded Rat cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

  • Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/5000 dilution (purified) + B16F0 (Mouse melanoma cell line) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa

    Blocking  and Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin embedded Mouse cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

  • Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/5000 dilution (purified) + A-375 (Human malignant melanoma cell line) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

  • S100 beta was immunoprecipitated from Human fetal brain with purified ab52642 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52642 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

  • Unpurified ab52642 staining S100 beta in Human spiral ganglion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/200 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of embryonic mouse brain tissue, staining S100 beta with unpurified ab52642.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-S100 beta antibody [EP1576Y] (ab52642)

This product has been referenced in:
  • Jia X  et al. Large cervicothoracic myxoinflammatory fibroblastic sarcoma with brachial plexus invasion: A case report and literature review. Oncol Lett 12:1717-1720 (2016). IHC-P ; Human . Read more (PubMed: 27588121) »
  • Liu W  et al. Hepatic epithelioid angiomyolipoma is a rare and potentially severe but treatable tumor: A report of three cases and review of the literature. Oncol Lett 11:3669-3675 (2016). IHC ; Human . Read more (PubMed: 27313680) »

See all 26 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (large intestine)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Permeabilization Yes - 0.1% Triton X-100
Specification large intestine
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
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Submitted May 16 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (large intestine)
Permeabilization Yes - 0.1% Triton X-100
Specification large intestine
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted May 16 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Adipose)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
Permeabilization No
Specification Adipose
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered Formalin
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Submitted Aug 20 2015

Application Immunocytochemistry
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Human Cell (Adipose tissue)
Specification Adipose tissue
Permeabilization Yes - 0.3%TritonX-100
Fixative Paraformaldehyde
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Verified customer

Submitted Mar 16 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde
Username

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Verified customer

Submitted Jan 26 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Sample Human Tissue sections (colon cancer tissue specimen)
Specification colon cancer tissue specimen
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 26 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Sample Mouse Tissue sections (colon)
Specification colon
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 30 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Goat Tissue sections (Small intestine)
Specification Small intestine
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 11 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Cat Tissue sections (Small intestine)
Specification Small intestine
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 11 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 11 2014

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