The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 100 µl working solution / test. Formaldehyde-fixed tissue should be pre-treated with 0.1% pronase. Unmasking methods involving microwave or steam cooking are not recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
Tissue specificityAlthough predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Sequence similaritiesBelongs to the S-101 family. Contains 2 EF-hand domains.