Recombinant
RabMAb

Anti-S100 Calcium Binding Protein A13 antibody [EPR4510] (ab109252)

Overview

  • Product name
    Anti-S100 Calcium Binding Protein A13 antibody [EPR4510]
    See all S100 Calcium Binding Protein A13 primary antibodies
  • Description
    Rabbit monoclonal [EPR4510] to S100 Calcium Binding Protein A13
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human S100 Calcium Binding Protein A13.

  • Positive control
    • Human thyroid and testis, HUVEC and ECV304 cell lysates; Human brain and muscle tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of a HRP/AP polymerized antibody recommended.

ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine.
  • Tissue specificity
    Expressed in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization
    Cytoplasm. Secreted. Secretion is mediated by exposure to stress and requires copper ions.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein S100 A13 antibody
    • Protein S100-A13 antibody
    • S100 calcium binding protein A13 antibody
    • S100 calcium-binding protein A13 antibody
    • S100a13 antibody
    • S10AD_HUMAN antibody
    see all

Images

  • All lanes : Anti-S100 Calcium Binding Protein A13 antibody [EPR4510] (ab109252) at 1/1000 dilution

    Lane 1 : Human thyroid tissue lysate
    Lane 2 : Human testis tissue lysate
    Lane 3 : HUVEC lysate
    Lane 4 : ECV304 lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 11 kDa
  • ab109252, at a 1/250 dilution, staining S100 Calcium Binding Protein A13 in Human brain, by Immunohistochemistry, Paraffin-embedded tissue.
  • ab109252, at a 1/250 dilution, staining S100 Calcium Binding Protein A13 in Human muscle, by Immunohistochemistry, Paraffin-embedded tissue.

References

ab109252 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109252.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up