Recombinant
RabMAb

Anti-S100A11 antibody [EPR11171(B)] (ab169530)

Overview

  • Product name
    Anti-S100A11 antibody [EPR11171(B)]
    See all S100A11 primary antibodies
  • Description
    Rabbit monoclonal [EPR11171(B)] to S100A11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human S100A11 (UniProt P31949).

  • Positive control
    • SKBR3, BxPC-3, PC-3 and HACAT cell lysates; BxPC-3 cells; HACAT cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 11 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Facilitates the differentiation and the cornification of keratinocytes.
    • Sequence similarities
      Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • Post-translational
      modifications
      Phosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calgizzarin antibody
      • Epididymis secretory protein Li 43 antibody
      • HEL S 43 antibody
      • Metastatic lymph node gene 70 protein antibody
      • MLN 70 antibody
      • MLN70 antibody
      • Protein S100 A11 antibody
      • Protein S100-A11, N-terminally processed antibody
      • Protein S100-C antibody
      • Protein S100A11 antibody
      • Protein S100C antibody
      • S100 A11 antibody
      • S100 calcium binding protein A11 antibody
      • S100 calcium-binding protein A11 (calgizzarin) antibody
      • S100 calcium-binding protein A11 antibody
      • S100a11 antibody
      • S100C antibody
      • S10AB_HUMAN antibody
      see all

    Images

    • All lanes : Anti-S100A11 antibody [EPR11171(B)] (ab169530) at 1/1000 dilution

      Lane 1 : SKBR3 cell lysate
      Lane 2 : BxPC-3 cell lysate
      Lane 3 : PC-3 cell lysate
      Lane 4 : HACAT cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 11 kDa

    • Immunofluorescent analysis of BxPC-3 cells labeling S100A11 with ab169530 at 1/250 dilution. DAPI nuclear staining (blue).
    • Flow cytometric analysis of permeabilized HACAT cells labeling S100A11 with ab169530 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    References

    ab169530 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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