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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
Tissue specificity Ubiquitously expressed.
Sequence similarities Belongs to the S-100 family. Contains 2 EF-hand domains.
Information by UniProt
calcium Placental protein antibody
Anti-S100A4 antibody images
Western blot - S100A4 antibody (ab58597)
Anti-S100A4 antibody (ab58597) at 0.03 µg/ml + Peripheral blood mononuclear cell lysate (in RIPA buffer) at 35 µg Predicted band size : 12 kDa Observed band size : 13 kDa ( why is the actual band size different from the predicted?) Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody (ab58597)
ab58597 at a concentration of 2µg/ml staining S100A4 in l
ymphatic cells in the lamina propria of human small intestine tissue sections by immunohistochemistry (paraffin-embedded). Antigen retrieval was with a citrate buffer.
References for Anti-S100A4 antibody (ab58597)
This product has been referenced in:
et al. Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target. Mol Cell Proteomics 12:3778-92 (2013).
Read more (PubMed: 24037664) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"