Overview

  • Product nameAnti-S100A4 antibody
    See all S100A4 primary antibodies
  • Description
    Mouse monoclonal to S100A4
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-102 (MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT) of Human S100A4 (AAH16300) with a proprietary tag.

  • Positive control
    • HeLa cell and NIH/3T3 lysates. Transfected 293T cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab93283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 12 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18A2 antibody
    • 42A antibody
    • calcium Placental protein antibody
    • Calvasculin antibody
    • CAPL antibody
    • Fibroblast specific protein 1 antibody
    • Fibroblast specific protein antibody
    • FSP1 antibody
    • Leukemia multidrug resistance associated protein antibody
    • Malignant transformation suppression 1 antibody
    • Metastasin antibody
    • MTS1 antibody
    • OTTHUMP00000015467 antibody
    • OTTHUMP00000015468 antibody
    • P9KA antibody
    • PEL98 antibody
    • Placental calcium-binding protein antibody
    • Protein Mts1 antibody
    • Protein S100 A4 antibody
    • Protein S100-A4 antibody
    • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
    • S100 calcium binding protein A4 antibody
    • S100 calcium-binding protein A4 antibody
    • S100a4 antibody
    • S10A4_HUMAN antibody
    see all

Anti-S100A4 antibody images

  • Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate
    Developed using the ECL technique

    Predicted band size : 12 kDa
    Observed band size : 37.22 kDa (why is the actual band size different from the predicted?)
  • Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + HeLa cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate
    Developed using the ECL technique

    Predicted band size : 12 kDa
  • Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + NIH/3T3 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate
    Developed using the ECL technique

    Predicted band size : 12 kDa
  • All lanes : Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer)

    Lane 1 : cell lysate from S100A4 transfected 293T cell line
    Lane 2 : cell lysate from non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate
    Developed using the ECL technique

    Predicted band size : 12 kDa
    Observed band size : 11.7 kDa (why is the actual band size different from the predicted?)
  • ab93283 at 15µg/ml staining S100A4 in fixed and permeabilised HeLa cells by immunofluorescence.
  • ab93283 detecting recombinant S100A4 (with a proprietary tag) by sandwich ELISA. The detection limit for recombinant S100A4 (with a proprietary tag) is approximately 0.1 ng/ml as a capture antibody.

References for Anti-S100A4 antibody (ab93283)

This product has been referenced in:
  • Wilding C  et al. ?-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. PLoS One 9:e90737 (2014). Read more (PubMed: 24595072) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (differentiated cell)
Specification differentiated cell
Fixative Paraformaldehyde
Permeabilization Yes - TritonX
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Nov 12 2012

Thank you for contacting us and your interest in our products.

I have looked into the three antibodies we have directed against S100A4 and whether they are likely to work when used in a sandwich ELISA. We have the following antibodies directe...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"