Overview

  • Product nameAnti-S100A4 antibody [EPR2761(2)]
    See all S100A4 primary antibodies
  • Description
    Rabbit monoclonal [EPR2761(2)] to S100A4
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human S100A4 aa 50 to the C-terminus (C terminal).
    Database link: P26447

  • Positive control
    • Human tonsil, A549, A375 and Human small intestine lysates; Human tonsil tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-S100A4 antibody (Alexa Fluor® 488) [EPR2761(2)] (ab196380)
    Anti-S100A4 antibody (Alexa Fluor® 647) [EPR2761(2)] (ab196168)

    Anti-S100A4 antibody (HRP) [EPR2761(2)] (ab195523)

Properties

Applications

Our Abpromise guarantee covers the use of ab124805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/80 - 1/800.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18A2 antibody
    • 42A antibody
    • calcium Placental protein antibody
    • Calvasculin antibody
    • CAPL antibody
    • Fibroblast specific protein 1 antibody
    • Fibroblast specific protein antibody
    • FSP1 antibody
    • Leukemia multidrug resistance associated protein antibody
    • Malignant transformation suppression 1 antibody
    • Metastasin antibody
    • MTS1 antibody
    • OTTHUMP00000015467 antibody
    • OTTHUMP00000015468 antibody
    • P9KA antibody
    • PEL98 antibody
    • Placental calcium-binding protein antibody
    • Protein Mts1 antibody
    • Protein S100 A4 antibody
    • Protein S100-A4 antibody
    • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
    • S100 calcium binding protein A4 antibody
    • S100 calcium-binding protein A4 antibody
    • S100a4 antibody
    • S10A4_HUMAN antibody
    see all

Anti-S100A4 antibody [EPR2761(2)] images

  • Unpurified ab124805 staining S100A4 in the A549 cell line from Human lungs by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with Triton X-100 0.25% in PBS. Samples were incubated with primary antibody (1/100) for 45 minutes at 25°C. A TRITC-conjugated Goat anti-rabbit  polyclonal was used as the secondary antibody.

    See Abreview

  • ab124805 staining S100A4 in HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 80% methanol and permeabilised with 0.1% Triton X-100 (in PBS). The sample was incubated with the primary antibody at a dilution of 1/800. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: ab172730 rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • ab124805 staining S100A4 in HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 80% methanol and permeabilised with 0.1% Triton X-100 (in PBS). The sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: ab172730 rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • ab124805 staining S100A4 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • All lanes : Anti-S100A4 antibody [EPR2761(2)] (ab124805) at 1/1000 dilution (unpurified)

    Lane 1 : Human tonsil lysate
    Lane 2 : A549 cell lysate
    Lane 3 : A375 cell lysate
    Lane 4 : Human small intestine lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • ab124805 immunoprecipitating S100A4. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a dilution of 1/1500.

    Lane 1: Human tonsil whole cell lysate (10ug)
    Lane 2: Human tonsil whole cell lysate 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124805 in human tonsil whole cell lysate

  • Anti-S100A4 antibody [EPR2761(2)] (ab124805) at 1000 + A549 (human lung carcinoma) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 12 kDa
    Additional bands at : 12 kDa. We are unsure as to the identity of these extra bands.

    Blocking buffer: 5% NFDM /TBST 

    Diluting Buffer: 5% NFDM /TBST 

  • All lanes : Anti-S100A4 antibody [EPR2761(2)] (ab124805) at 1/2500 dilution

    Lane 1 : Human tonsil tissue lysate
    Lane 2 : A375 (human malignant melanoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 12 kDa
    Additional bands at : 12 kDa. We are unsure as to the identity of these extra bands.

    Blocking Buffer: 5% NFDM /TBST

    Diluting Buffer: 5% NFDM /TBST 

  • Unpurified ab124805, at 1/250 dilution, staining S100A4 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

  • Immunohistochemical analysis of Human lung tissue, staining S100A4 with unpurified ab124805.

    Tissue was fixed with HOPE and blocked with blocking solution for 5 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

  • ab124805 staining S100A4 in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/500. ab7291 and ab150120 were used as counterstains for primary antibody ab124805 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

References for Anti-S100A4 antibody [EPR2761(2)] (ab124805)

This product has been referenced in:
  • Katanov C  et al. Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-a and the NF-?B pathway. Stem Cell Res Ther 6:87 (2015). WB ; Human . Read more (PubMed: 25928089) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 23°C
Fixative Acetone
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Abcam user community

Verified customer

Submitted Sep 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (A549)
Specification A549
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Triton X-100/PBS
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Abcam user community

Verified customer

Submitted Apr 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung tissue)
Specification Lung tissue
Fixative HOPE
Antigen retrieval step None
Permeabilization No
Blocking step Zytomed Systems HRP Polymer Block Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Jan 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"