Recombinant
RabMAb

Anti-S100A4 antibody [EPR2761(2)] (HRP) (ab195523)

Overview

  • Product name
    Anti-S100A4 antibody [EPR2761(2)] (HRP)
    See all S100A4 primary antibodies
  • Description
    Rabbit monoclonal [EPR2761(2)] to S100A4 (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human S100A4 (C terminal). The exact sequence is proprietary.

  • Positive control
    • IHC-P: human normal lung tissue sections.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab195523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18A2 antibody
    • 42A antibody
    • calcium Placental protein antibody
    • Calvasculin antibody
    • CAPL antibody
    • Fibroblast specific protein 1 (FSP1) antibody
    • Fibroblast specific protein 1 antibody
    • Fibroblast specific protein antibody
    • FSP1 antibody
    • Leukemia multidrug resistance associated protein antibody
    • Malignant transformation suppression 1 (MTS1) antibody
    • Malignant transformation suppression 1 antibody
    • Metastasin antibody
    • MTS1 antibody
    • OTTHUMP00000015467 antibody
    • OTTHUMP00000015468 antibody
    • P9KA antibody
    • PEL98 antibody
    • Placental calcium-binding protein antibody
    • Protein Mts1 antibody
    • Protein S100 A4 antibody
    • Protein S100-A4 antibody
    • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
    • S100 calcium binding protein A4 antibody
    • S100 calcium-binding protein A4 antibody
    • S100a4 antibody
    • S10A4_HUMAN antibody
    see all

Images

  • IHC image of S100A4 staining in a section of formalin-fixed paraffin-embedded human normal lung*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab195523 at a dilution of 1 in 500. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

References

ab195523 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab195523.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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