Overview

  • Product nameAnti-S100A4 antibody [NJ4F3]
    See all S100A4 primary antibodies
  • Description
    Mouse monoclonal [NJ4F3] to S100A4
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, Competitive ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Genetic immunisation with cDNA encoding S100A4.

Properties

Applications

Our Abpromise guarantee covers the use of ab68124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1.2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Competitive ELISA 1/200 - 1/400.
ELISA 1/200 - 1/400.

Target

  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18A2 antibody
    • 42A antibody
    • calcium Placental protein antibody
    • Calvasculin antibody
    • CAPL antibody
    • Fibroblast specific protein 1 antibody
    • Fibroblast specific protein antibody
    • FSP1 antibody
    • Leukemia multidrug resistance associated protein antibody
    • Malignant transformation suppression 1 antibody
    • Metastasin antibody
    • MTS1 antibody
    • OTTHUMP00000015467 antibody
    • OTTHUMP00000015468 antibody
    • P9KA antibody
    • PEL98 antibody
    • Placental calcium-binding protein antibody
    • Protein Mts1 antibody
    • Protein S100 A4 antibody
    • Protein S100-A4 antibody
    • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
    • S100 calcium binding protein A4 antibody
    • S100 calcium-binding protein A4 antibody
    • S100a4 antibody
    • S10A4_HUMAN antibody
    see all

Anti-S100A4 antibody [NJ4F3] images

  • FACS analysis of BOSC23 cells, using ab68124 at 1.2µg for 106 cells. BOSC23 cells were transiently transfected with an expression vector encoding either S100 A4 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of NJ-4F3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with S100 A4 transfected cells.
  • ICC/IF image of ab68124 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68124, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-S100A4 antibody [NJ4F3] (ab68124)

This product has been referenced in:
  • Clements D  et al. Wild type mesenchymal cells contribute to the lung pathology of lymphangioleiomyomatosis. PLoS One 10:e0126025 (2015). IF ; Human . Read more (PubMed: 25978616) »
  • Scheiter M  et al. Proteome analysis of distinct developmental stages of human natural killer (NK) cells. Mol Cell Proteomics 12:1099-114 (2013). Human . Read more (PubMed: 23315794) »

See all 2 Publications for this product

Product Wall

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