Anti-S100A9 antibody [2B10] (ab105472)
Key features and details
- Rat monoclonal [2B10] to S100A9
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-S100A9 antibody [2B10]
See all S100A9 primary antibodies -
Description
Rat monoclonal [2B10] to S100A9 -
Host species
Rat -
Specificity
Specific for mouse S100-A9 protein that forms a heterodimer with S100-A8 and constitutes 45% of the neutrophil cytosolic protein. Stains neutrophils strongly and monocytes weakly. -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein, corresponding to amino acids 1-113 of Mouse S100A9
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Positive control
- Neutrophils FFPE mouse lung tissue sections RAW246.7 cell line
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General notes
Growth Conditions: RPMI 2% + 10% FCS and weaned down to 5% FCSThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2B10 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions. -
Tissue specificity
Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylated. Phosphorylation inhibits activation of tubulin polymerization. -
Cellular localization
Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation. - Information by UniProt
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Database links
- Entrez Gene: 6280 Human
- Entrez Gene: 20202 Mouse
- Omim: 123886 Human
- SwissProt: P06702 Human
- SwissProt: P31725 Mouse
- Unigene: 112405 Human
- Unigene: 2128 Mouse
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Alternative names
- Leukocyte L1 complex heavy chain antibody
- 60B8AG antibody
- CAGB antibody
see all
Images
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IHC image of S100A9 staining in mouse lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab105472, 10µg/ml, for 15 mins at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
ICC/IF image of ab105472 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105472, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab98386, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab105472 has been referenced in 22 publications.
- Baluk P & McDonald DM Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts. Methods Mol Biol 2441:115-134 (2022). PubMed: 35099733
- Zhao B et al. S100A9 blockade prevents lipopolysaccharide-induced lung injury via suppressing the NLRP3 pathway. Respir Res 22:45 (2021). PubMed: 33549095
- Dalal PJ et al. Spatiotemporal restriction of endothelial cell calcium signaling is required during leukocyte transmigration. J Exp Med 218:N/A (2021). PubMed: 32970800
- Liao YL et al. S100A9 Upregulation Contributes to Learning and Memory Impairments by Promoting Microglia M1 Polarization in Sepsis Survivor Mice. Inflammation 44:307-320 (2021). PubMed: 32918665
- Crespo M et al. Neutrophil infiltration regulates clock-gene expression to organize daily hepatic metabolism. Elife 9:N/A (2020). PubMed: 33287957