• Product nameAnti-S100A9 antibody [47-8D3]
    See all S100A9 primary antibodies
  • Description
    Mouse monoclonal [47-8D3] to S100A9
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation of peripheral blood monocyte components (Human).

  • Positive control
    • Lysate of human monocytes or granulocytes.
  • General notes

    Can be used for phenotypical characterization of macrophages and myelomonocytic cells in situ.



Our Abpromise guarantee covers the use of ab24111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 14 kDa.


  • FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • Tissue specificityExpressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Post-translational
    Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
  • Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Leukocyte L1 complex heavy chain antibody
    • 60B8AG antibody
    • CAGB antibody
    • Calgranulin B antibody
    • Calgranulin-B antibody
    • Calprotectin L1H subunit antibody
    • CFAG antibody
    • CGLB antibody
    • Cystic fibrosis antigen B antibody
    • L1AG antibody
    • Leukocyte L1 complex heavy chain antibody
    • LIAG antibody
    • MAC387 antibody
    • MIF antibody
    • Migration inhibitory factor related protein 14 antibody
    • Migration inhibitory factor-related protein 14 antibody
    • MRP 14 antibody
    • MRP-14 antibody
    • MRP14 antibody
    • Myeloid-related protein 14 antibody
    • NIF antibody
    • OTTHUMP00000015331 antibody
    • p14 antibody
    • Protein S100-A9 antibody
    • S100 A9 antibody
    • S100 calcium binding protein A9 antibody
    • S100 calcium binding protein A9 calgranulin B antibody
    • S100 calcium-binding protein A9 antibody
    • S100A9 antibody
    • S10A9_HUMAN antibody
    see all

References for Anti-S100A9 antibody [47-8D3] (ab24111)

This product has been referenced in:
  • Gschwandtner M  et al. Fetal Human Keratinocytes Produce Large Amounts of Antimicrobial Peptides: Involvement of Histone-Methylation Processes. J Invest Dermatol N/A:N/A (2014). Read more (PubMed: 24694903) »
  • Gromov P  et al. Up-regulated proteins in the fluid bathing the tumour cell microenvironment as potential serological markers for early detection of cancer of the breast. Mol Oncol 4:65-89 (2010). IHC-P ; Human . Read more (PubMed: 20005186) »

See all 3 Publications for this product

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We do not have this type of clinical data as our products are for in vitro use. A literature search should be able to provide you with this kind of information.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Oral dyplastic epithelium cells)
Specification Oral dyplastic epithelium cells
Fixative Paraformaldehyde
Antigen retrieval step Enzymatic
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2%

Mr. Cancer center

Verified customer

Submitted Jul 25 2006

Thank you for your enquiry. The antibody reacts with MRP 14 (L1 monocyte antigen), the exact epitope is not known. I hope this information helps. Please do not hesitate to contact us again if you need anyting further.