Overview

  • Product nameAnti-S100A9 antibody
    See all S100A9 primary antibodies
  • Description
    Rabbit polyclonal to S100A9
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human S100A9.

    (Peptide available as ab74434.)

  • Positive control
    • This antibody gave a positive signal in the following Human Tissue Lysates: Lymph node, Thymus, Spleen, Lung This antibody gave a positive result in IHC in the following FFPE tissue: Human Tonsil.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab63818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • Tissue specificityExpressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
  • Sequence similaritiesBelongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
  • Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Leukocyte L1 complex heavy chain antibody
    • 60B8AG antibody
    • CAGB antibody
    • Calgranulin B antibody
    • Calgranulin-B antibody
    • Calprotectin L1H subunit antibody
    • CFAG antibody
    • CGLB antibody
    • Cystic fibrosis antigen B antibody
    • L1AG antibody
    • Leukocyte L1 complex heavy chain antibody
    • LIAG antibody
    • MAC387 antibody
    • MIF antibody
    • Migration inhibitory factor related protein 14 antibody
    • Migration inhibitory factor-related protein 14 antibody
    • MRP 14 antibody
    • MRP-14 antibody
    • MRP14 antibody
    • Myeloid-related protein 14 antibody
    • NIF antibody
    • OTTHUMP00000015331 antibody
    • p14 antibody
    • Protein S100-A9 antibody
    • S100 A9 antibody
    • S100 calcium binding protein A9 antibody
    • S100 calcium binding protein A9 calgranulin B antibody
    • S100 calcium-binding protein A9 antibody
    • S100A9 antibody
    • S10A9_HUMAN antibody
    see all

Anti-S100A9 antibody images

  • IHC image of S100A9 staining in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63818, 0.1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-S100A9 antibody (ab63818) at 1 µg/ml

    Lane 1 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 13 kDa
    Observed band size : 13 kDa


    Exposure time : 5 minutes
  • ICC/IF image of ab63818 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63818, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and Hek293 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, HepG2, Hek293 and MCF7 cells at 5µg/ml.

References for Anti-S100A9 antibody (ab63818)

ab63818 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization No
Specification spleen
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 21°C
Fixative 10% NBF
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Submitted Oct 25 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (lymph node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization No
Specification lymph node
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative Formaldehyde
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Submitted Dec 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Burkitt's lymphoma cell line xenograft)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization No
Specification Burkitt's lymphoma cell line xenograft
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative Formaldehyde
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Submitted Dec 18 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human neutrophil cell lysate)
Loading amount 100 µg
Specification human neutrophil cell lysate
Gel Running Conditions Reduced Denaturing (12.5% of gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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Submitted Dec 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"