Anti-S100A9 antibody (ab75478)


  • Product name
  • Description
    Rabbit polyclonal to S100A9
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length human S100A9 protein [Swiss-Prot P06702] expressed in E. coli.

  • Positive control
    • DMSO treated HL60 whole cell lysate



Our Abpromise guarantee covers the use of ab75478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 16 kDa.


  • Function
    Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • Tissue specificity
    Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
  • Sequence similarities
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Post-translational
    Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
  • Cellular localization
    Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Leukocyte L1 complex heavy chain antibody
    • 60B8AG antibody
    • CAGB antibody
    • Calgranulin B antibody
    • Calgranulin-B antibody
    • Calprotectin L1H subunit antibody
    • CFAG antibody
    • CGLB antibody
    • Cystic fibrosis antigen B antibody
    • L1AG antibody
    • Leukocyte L1 complex heavy chain antibody
    • LIAG antibody
    • MAC387 antibody
    • MIF antibody
    • Migration inhibitory factor related protein 14 antibody
    • Migration inhibitory factor-related protein 14 antibody
    • MRP 14 antibody
    • MRP-14 antibody
    • MRP14 antibody
    • Myeloid-related protein 14 antibody
    • NIF antibody
    • OTTHUMP00000015331 antibody
    • p14 antibody
    • Protein S100-A9 antibody
    • S100 A9 antibody
    • S100 calcium binding protein A9 antibody
    • S100 calcium binding protein A9 calgranulin B antibody
    • S100 calcium-binding protein A9 antibody
    • S100A9 antibody
    • S10A9_HUMAN antibody
    see all

Anti-S100A9 antibody images

References for Anti-S100A9 antibody (ab75478)

ab75478 has not yet been referenced specifically in any publications.

Product Wall

Western blot
Mouse Tissue lysate - whole (liver, spleen, etc.)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
15 µg
liver, spleen, etc.
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 18 2017

Thank you for your reply.

I agree that the Researchgate forum seems to be more helpful. Please keep me updated about any new results, and let me know if you have further questions.

Have a great day!

Thank you again for your reply and for sending the additional information.

I have confirmed with my contact in the lab that they also see bands around the same sizes that you have mentioned. The bands unfortunately have not been characterized...

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Thank you very much for your reply.

I am glad to hear that the results have been a little better. I will double-check with the lab about the sizes of the extra bands that have been seen on the blots.

Does your sample buffer contain ...

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Thank you for your call this week and for your patience while I have been in touch with the lab.

The lab has also seen similar non-specific bands in Western blot, and my colleague recommends increasing the membrane washing times, further dilu...

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My colleague Anja is on tour for Abcam this week and therefore I am dealing with this case. I apologize for the delay of my reply! Thank you very much for confirming these details for us. Looking at the previous correspondence, it appears that - ...

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