Anti-S100A9 antibody (ab81522)

Overview

• Product name
  Anti-S100A9 antibody
  See all S100A9 primary antibodies
• Description
  Goat polyclonal to S100A9
• Tested applications
  Suitable for: WB, ELISAmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Rabbit, Cow
• Immunogen

  Synthetic peptide:

  C-DTNADKQLSFEEF

  , corresponding to internal sequence amino acids 67-79 of Human S100A9 according to NP_002956.1

  Run BLAST with Run BLAST with
• Positive control
  • Human Peripheral Mononucleocytes lysate

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris saline, pH 7.3
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Purification notes
  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Calcium Binding Proteins

  • Cancer
  • Tumor biomarkers
  • Other

Associated products

• Compatible Secondaries
  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
• Positive Controls
  • Recombinant Human S100A9 protein (ab95909)

Applications

Target

• Function
  Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
• Tissue specificity
  Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
• Sequence similarities
  Belongs to the S-100 family.
  Contains 2 EF-hand domains.
• Post-translational
  modifications
  Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
• Cellular localization
  Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.

• Target information above from: UniProt accession P06702 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 6280 Human
  • Omim: 123886 Human
  • SwissProt: P06702 Human
  • Unigene: 112405 Human
• Alternative names
  • Leukocyte L1 complex heavy chain antibody
  • 60B8AG antibody
  • CAGB antibody

  • Calgranulin B antibody
  • Calgranulin-B antibody
  • Calprotectin L1H subunit antibody
  • CFAG antibody
  • CGLB antibody
  • Cystic fibrosis antigen B antibody
  • L1AG antibody
  • Leukocyte L1 complex heavy chain antibody
  • LIAG antibody
  • MAC387 antibody
  • MIF antibody
  • Migration inhibitory factor related protein 14 antibody
  • Migration inhibitory factor-related protein 14 antibody
  • MRP 14 antibody
  • MRP-14 antibody
  • MRP14 antibody
  • Myeloid-related protein 14 antibody
  • NIF antibody
  • OTTHUMP00000015331 antibody
  • p14 antibody
  • Protein S100-A9 antibody
  • S100 A9 antibody
  • S100 calcium binding protein A9 antibody
  • S100 calcium binding protein A9 calgranulin B antibody
  • S100 calcium-binding protein A9 antibody
  • S100A9 antibody
  • S10A9_HUMAN antibody

  see all

Protocols

• Western blot protocols