The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 2µg for 106 cells. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
Tissue specificityExpressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
Sequence similaritiesBelongs to the S-100 family. Contains 2 EF-hand domains.
Post-translational modificationsPhosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
Tissue Distribution by Flow Cytometry Analysis:
Human peripheral blood leukocytes labeled with ab112210 at a concentration of 2.0 µg for 106 cells, and PE secondary antibody.
Percentage of cells stained above control: 74.7%
Isotypic Control (shaded): Purified Mouse IgG1
References for Anti-S100A9 antibody [CF-557] (Phycoerythrin) (ab112210)
has not yet been referenced specifically in any publications.
Publishing research using ab112210? Please let us know so that we can cite the reference in this datasheet.
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