Overview

  • Product nameAnti-S100A9 antibody [EPR3555]
    See all S100A9 primary antibodies
  • Description
    Rabbit monoclonal [EPR3555] to S100A9
  • Tested applicationsSuitable for: WB, IHC-P, ICC, IHC-Frmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human S100A9 aa 1-100.

  • Positive control
    • Breast cancer, Human tonsil, Human spleen and peripheral blood lymphocytes lysates, Human breast carcinoma tissue and Human spleen tissue. IF: DU145 cell line
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-S100A9 antibody (Alexa Fluor® 488) [EPR3555] (ab200972)

    Anti-S100A9 antibody (Alexa Fluor® 647) [EPR3555] (ab200974)

Properties

Applications

Our Abpromise guarantee covers the use of ab92507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 13 kDa.
IHC-P 1/250 - 1/500.
ICC 1/100 - 1/250.
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
    • Tissue specificityExpressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
    • Sequence similaritiesBelongs to the S-100 family.
      Contains 2 EF-hand domains.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
    • Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
    • Information by UniProt
    • Database links
    • Alternative names
      • Leukocyte L1 complex heavy chain antibody
      • 60B8AG antibody
      • CAGB antibody
      • Calgranulin B antibody
      • Calgranulin-B antibody
      • Calprotectin L1H subunit antibody
      • CFAG antibody
      • CGLB antibody
      • Cystic fibrosis antigen B antibody
      • L1AG antibody
      • Leukocyte L1 complex heavy chain antibody
      • LIAG antibody
      • MAC387 antibody
      • MIF antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor-related protein 14 antibody
      • MRP 14 antibody
      • MRP-14 antibody
      • MRP14 antibody
      • Myeloid-related protein 14 antibody
      • NIF antibody
      • OTTHUMP00000015331 antibody
      • p14 antibody
      • Protein S100-A9 antibody
      • S100 A9 antibody
      • S100 calcium binding protein A9 antibody
      • S100 calcium binding protein A9 calgranulin B antibody
      • S100 calcium-binding protein A9 antibody
      • S100A9 antibody
      • S10A9_HUMAN antibody
      see all

    Anti-S100A9 antibody [EPR3555] images

    • All lanes : Anti-S100A9 antibody [EPR3555] (ab92507) at 1/1000 dilution

      Lane 1 : Breast cancer lysate
      Lane 2 : Human tonsil lysate
      Lane 3 : Human spleen lysate
      Lane 4 : Peripheral blood lymphocytes lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 13 kDa
      Observed band size : 14 kDa (why is the actual band size different from the predicted?)
    • ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
    • ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human spleen tissue by Immunohistochemistry.
    • ICC/IF image of ab92507 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92507, 1/200) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgM (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-S100A9 antibody [EPR3555] (ab92507)

    This product has been referenced in:
    • Saul MJ  et al. UPF1 regulates myeloid cell functions and S100A9 expression by the hnRNP E2/miRNA-328 balance. Sci Rep 6:31995 (2016). WB ; Human . Read more (PubMed: 27573788) »
    • Caseiro A  et al. Pursuing type 1 diabetes mellitus and related complications through urinary proteomics. Transl Res N/A:N/A (2013). Read more (PubMed: 24096133) »

    See all 3 Publications for this product

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    Thank you for contacting us. The concentration of lot number GR49053 is 0.431 mg/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"