Overview

  • Product name
  • Description
    Rabbit polyclonal to S1P
  • Specificity
    We have a range of domain specific antibodies for this target. For a full list please see all MBTPS1 antibodies. ab59870 recognizes all four forms of MBTPS1 (1052, 746, 607 and 552 amino acids in length).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the catalytic domain of human MBTPS1. Immunizing peptide available on request - please contact a member of staff to ask for availability.

Properties

Applications

Our Abpromise guarantee covers the use of ab59870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 118 kDa. 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates.

Target

  • Relevance
    S1P (also known as MBTPS1,SKI1), was first discovered as one of two enzymes that cleave the sterol regulatory binding proteins (SREBPs), in the case of S1P at “site-1,” a hydrophillic loop extending into the lumen of the ER. The MBTPS1 domain structure contains a signal sequence followed by a propeptide domain, a catalytic domain, a transmembrane domain and a cytoplasmic domain.
  • Cellular localization
    Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • kexin isozyme 1 antibody
    • KIAA0091 antibody
    • MBTPS1 antibody
    • membrane bound transcription factor peptidase, site 1 antibody
    • MGC138711 antibody
    • MGC138712 antibody
    • PCSK8 antibody
    • S1P antibody
    • S1P endopeptidase antibody
    • Site 1 protease antibody
    • SKI 1 antibody
    • SKI1 antibody
    • Subtilisin isozyme 1 antibody
    • Subtilisin/kexin isozyme 1 antibody
    see all

Images

  • Anti-S1P antibody (ab59870) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 118 kDa


    Exposure time : 16 minutes

References

ab59870 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 Cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
HepG2 Cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Dec 21 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Dec 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up