Overview

  • Product nameAnti-S2P antibody
    See all S2P primary antibodies
  • Description
    Mouse monoclonal to S2P
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Mouse, Cow, Human, Chinese Hamster
  • Immunogen

    Recombinant fragment: ASTLQQLSFP VRAYKRLDGS TECCNNHSLT DVCFSYRNNF NKRLHTCLPA RKAVEATQVC RTNKDCKKSS SSSFCIIPSL ETHTRLIKVK HPPQIDMLYV GHPLHLH, corresponding to amino acids 312-419 of human MBTPS2 (NP_056968) with a tag

  • Positive control
    • Partial recombinant human MBTPS2 protein (the immunogen)

Properties

Applications

Our Abpromise guarantee covers the use of ab76641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa.
    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIntramembrane proteolysis of sterol-regulatory element-binding proteins (SREBPs) within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop.
    • Tissue specificityExpressed in heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
    • Involvement in diseaseDefects in MBTPS2 are the cause of ichthyosis follicularis-atrichia-photophobia syndrome (IFAPS) [MIM:308205]. A syndrome characterized by a peculiar triad of follicular ichthyosis, total or subtotal atrichia, and photophobia of varying degree. Histopathologically, the epidermal granular layer is generally well-preserved or thickened at the infundibulum. Hair follicles are poorly developed and tend to be surrounded by an inflammatory infiltrate. A subgroup of patients is described with lamellar rather than follicular ichthyosis. Non-consistent features may include growth and psychomotor retardation, aganglionic megacolon, seizures and nail dystrophy.
      Defects in MBTPS2 are a cause of keratosis follicularis spinulosa decalvans X-linked (KFSDX) [MIM:308800]. A rare disorder affecting the skin and the eye. Affected men show thickening of the skin of the neck, ears, and extremities, especially the palms and soles, loss of eyebrows, eyelashes and beard, thickening of the eyelids with blepharitis and ectropion, and corneal degeneration.
    • Sequence similaritiesBelongs to the peptidase M50A family.
    • Cellular localizationMembrane. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • BRESEK antibody
      • Endopeptidase S2P antibody
      • IFAP antibody
      • KFSDX antibody
      • MBTP2_HUMAN antibody
      • MBTPS2 antibody
      • Membrane bound transcription factor site 2 protease antibody
      • membrane-bound transcription factor peptidase, site 2 antibody
      • membrane-bound transcription factor protease, site 2 antibody
      • Membrane-bound transcription factor site-2 protease antibody
      • S2P antibody
      • S2P endopeptidase antibody
      • Site 2 protease antibody
      • SREBPs intramembrane protease antibody
      • Sterol regulatory element-binding proteins intramembrane protease antibody
      see all

    Anti-S2P antibody images

    • Anti-S2P antibody (ab76641) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 57 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    References for Anti-S2P antibody (ab76641)

    ab76641 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76641.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"