Anti-S6K1 antibody [6B2] (ab119252)


  • Product nameAnti-S6K1 antibody [6B2]
    See all S6K1 primary antibodies
  • Description
    Mouse monoclonal [6B2] to S6K1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human S6K1.

  • Positive control
    • HEK293T cells transfected with the pCMV6-ENTRY S6K cDNA; COS7 cells transiently transfected by pCMV6-ENTRY S6K; Human Tonsil tissue; SVT2, COS7, MDCK, and MCF7 Cell extracts.
  • General notesDilute in PBS (pH7.3) before use.
    Stable for 12 months from date of receipt.



Our Abpromise guarantee covers the use of ab119252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 59 kDa.
IHC-P 1/150.
ICC/IF 1/100.


  • FunctionActs to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • DomainThe autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
    The TOS (TOR signaling) motif is essential for activation by mTORC1.
  • Post-translational
    Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
  • Cellular localizationCytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa ribosomal protein S6 kinase 1 antibody
    • KS6B1_HUMAN antibody
    • p70 alpha antibody
    • P70 beta 1 antibody
    • p70 ribosomal S6 kinase alpha antibody
    • p70 ribosomal S6 kinase beta 1 antibody
    • p70 S6 kinase alpha antibody
    • P70 S6 Kinase antibody
    • p70 S6 kinase, alpha 1 antibody
    • p70 S6 kinase, alpha 2 antibody
    • p70 S6K antibody
    • p70 S6K-alpha antibody
    • p70 S6KA antibody
    • p70(S6K) alpha antibody
    • p70(S6K)-alpha antibody
    • p70-alpha antibody
    • p70-S6K 1 antibody
    • p70-S6K antibody
    • P70S6K antibody
    • P70S6K1 antibody
    • p70S6Kb antibody
    • PS6K antibody
    • Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
    • Ribosomal protein S6 kinase beta 1 antibody
    • Ribosomal protein S6 kinase beta-1 antibody
    • Ribosomal protein S6 kinase I antibody
    • RPS6KB1 antibody
    • S6K antibody
    • S6K-beta-1 antibody
    • S6K1 antibody
    • Serine/threonine kinase 14 alpha antibody
    • Serine/threonine-protein kinase 14A antibody
    • STK14A antibody
    see all

Anti-S6K1 antibody [6B2] images

  • Predicted band size : 59 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: S6K1 knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab119252 observed at 68 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab119252 was shown to recognize S6K1 when S6K1 knockout samples were used, along with additional cross-reactive bands. Wild-type and S6K1 knockout samples were subjected to SDS-PAGE. ab119252 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-S6K1 antibody [6B2] (ab119252) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA.
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY S6K cDNA.

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 59 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203214)
  • All lanes : Anti-S6K1 antibody [6B2] (ab119252) at 1/2000 dilution

    Lane 1 : HepG2 Cell extract
    Lane 2 : HeLa Cell extract
    Lane 3 : SVT2 Cell extract
    Lane 4 : A549 Cell extract
    Lane 5 : COS7 Cell extract
    Lane 6 : Jurkat Cell extract
    Lane 7 : MDCK Cell extract
    Lane 8 : PC12 Cell extract
    Lane 9 : MCF7 Cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size : 59 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203214)
  • ab119252, at 1/150, staining S6K in paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
  • ab119252 at 1/100, staining S6K in COS7 cells transiently transfected with pCMV6-ENTRY S6K by Immunofluorescence.

References for Anti-S6K1 antibody [6B2] (ab119252)

ab119252 has not yet been referenced specifically in any publications.

Product Wall

I have had a response from the customer which purchased the ab119252. Unfortunately there was only one, and they have responded that they require all of the antibody themselves and are regrettably unable to share it.

I am very sorry for this....

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Thats no problem. Thank you for getting back to me with the answer.