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Synthetic peptide: MISKRPEHLRMNL, corresponding to amino acids 490-502 of S6K1.
Our Abpromise guarantee covers the use of ab14708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 65-70 kDa (predicted molecular weight: 60 kDa).Can be blocked with Human S6K1 peptide (ab23245).|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab14708 (2µg/ml) staining of paraffin embedded Human Kidney shows staining in the cytoplasm-rich PCT while less so in the DCT and no signals in the glomerulus. following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.
Western blot showing ab14708 staining (0.2
ab14708 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"