Overview

  • Product nameAnti-S6K1 antibody
    See all S6K1 primary antibodies
  • Description
    Rabbit polyclonal to S6K1
  • SpecificityThis antibody detects endogenous levels of total S6K protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic peptide derived from an internal region of human S6K.

  • Positive control
    • Human breast carcinoma and extracts from COLO205 and Jurkat cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab64804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ELISA
  • Application notesELISA: 1/5000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
    • DomainThe autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
      The TOS (TOR signaling) motif is essential for activation by mTORC1.
    • Post-translational
      modifications
      Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
    • Cellular localizationCytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 70 kDa ribosomal protein S6 kinase 1 antibody
      • KS6B1_HUMAN antibody
      • p70 alpha antibody
      • P70 beta 1 antibody
      • p70 ribosomal S6 kinase alpha antibody
      • p70 ribosomal S6 kinase beta 1 antibody
      • p70 S6 kinase alpha antibody
      • P70 S6 Kinase antibody
      • p70 S6 kinase, alpha 1 antibody
      • p70 S6 kinase, alpha 2 antibody
      • p70 S6K antibody
      • p70 S6K-alpha antibody
      • p70 S6KA antibody
      • p70(S6K) alpha antibody
      • p70(S6K)-alpha antibody
      • p70-alpha antibody
      • p70-S6K 1 antibody
      • p70-S6K antibody
      • P70S6K antibody
      • P70S6K1 antibody
      • p70S6Kb antibody
      • PS6K antibody
      • Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
      • Ribosomal protein S6 kinase beta 1 antibody
      • Ribosomal protein S6 kinase beta-1 antibody
      • Ribosomal protein S6 kinase I antibody
      • RPS6KB1 antibody
      • S6K antibody
      • S6K-beta-1 antibody
      • S6K1 antibody
      • Serine/threonine kinase 14 alpha antibody
      • Serine/threonine-protein kinase 14A antibody
      • STK14A antibody
      see all

    Anti-S6K1 antibody images

    • All lanes : Anti-S6K1 antibody (ab64804) at 1/500 dilution

      Lane 1 : Extracts of COLO205 cells
      Lane 2 : Extracts of Jurkat cells
      Lane 3 : Extracts of Jurkat cells plus peptide

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 59 kDa
      Observed band size : 59 kDa
    • Immunohistochemical analysis of S6K in paraffin embedded human breast carcinoma tissue using ab64804 at a 1/50 dilution.
      Left image untreated.
      Right image treated with peptide.

    References for Anti-S6K1 antibody (ab64804)

    This product has been referenced in:
    • Zeng R  et al. The time-dependent autophagy protects against apoptosis with possible involvement of Sirt1 protein in multiple myeloma under nutrient depletion. Ann Hematol 91:407-17 (2012). WB ; Human . Read more (PubMed: 21915620) »

    See 1 Publication for this product

    Product Wall

    Thank you for your reply.

    Unfortunately, we do not offer free or trial sized samples of any of our products. If you would like to test ab64804, we do offer a 100% testing discount program. If you purchase the antibody for full price and submi...

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    Thank you for your patience.

    I checked the remaining antibodies we have, and the best match for total S6K would be ab64804. The immunogen is 76% homologous with drosophila, and the antibody is polyclonal so it should recognize multiple epitop...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"