Overview

  • Product nameAnti-S6K1 antibody
    See all S6K1 primary antibodies
  • Description
    Rabbit polyclonal to S6K1
  • SpecificityReacts strongly with 70 kd of p70 S6K, but only weakly with the 80 kDa S6K.
  • Tested applicationsWB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cow, Human, Pig
  • Immunogen

    S6K c-terminal peptide conjugated to KLH and BSA.

  • EpitopeThe antigen is based on the C-terminal sequence of human p70 S6K (C-ISKRPEHLRMNL).
  • Positive control
    • Use Mouse brain extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab9366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250.
ELISA 1/500.
IP Use at 3 µg/mg of lysate.

Target

  • FunctionActs to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • DomainThe autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
    The TOS (TOR signaling) motif is essential for activation by mTORC1.
  • Post-translational
    modifications
    Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
  • Cellular localizationCytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa ribosomal protein S6 kinase 1 antibody
    • KS6B1_HUMAN antibody
    • p70 alpha antibody
    • P70 beta 1 antibody
    • P70 beta 1 antibody
    • p70 ribosomal S6 kinase alpha antibody
    • p70 ribosomal S6 kinase beta 1 antibody
    • p70 ribosomal S6 kinase beta 1 antibody
    • p70 S6 kinase alpha antibody
    • P70 S6 Kinase antibody
    • p70 S6 kinase, alpha 1 antibody
    • p70 S6 kinase, alpha 2 antibody
    • p70 S6K antibody
    • p70 S6K-alpha antibody
    • p70 S6KA antibody
    • p70(S6K) alpha antibody
    • p70(S6K)-alpha antibody
    • p70-alpha antibody
    • p70-S6K 1 antibody
    • p70-S6K antibody
    • P70S6K antibody
    • P70S6K1 antibody
    • p70S6Kb antibody
    • p70S6Kb antibody
    • PS6K antibody
    • Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
    • Ribosomal protein S6 kinase 70kDa polypeptide 2 antibody
    • Ribosomal protein S6 kinase 70kDa polypeptide 2 antibody
    • Ribosomal protein S6 kinase beta 1 antibody
    • Ribosomal protein S6 kinase beta-1 antibody
    • Ribosomal protein S6 kinase I antibody
    • RPS6KB1 antibody
    • RPS6KB2 antibody
    • RPS6KB2 antibody
    • S6K antibody
    • S6K-beta-1 antibody
    • S6K1 antibody
    • Serine/threonine kinase 14 alpha antibody
    • Serine/threonine-protein kinase 14A antibody
    • STK14A antibody
    see all

Anti-S6K1 antibody images

  • One-step immunoblotting with cross-antibodies of the immunoprecipitated kinases
    Immunoprecipitation with anti-p90 RSK1 (Lane A and C)
    Immunoprecipitation with anti-p70 S6K (Lane B and D)
    Immunoblotting with anti-p70 S6K HRPconjugates (Lane A and B)
    Immunoblotting with anti-RSK1 HRP conjugates (Lane C and D)
  • One-step immunoblotting of crude mouse brain and muscle samples with anti-S6K HRP conjugates (ECL system).
    Lane A: mouse Muscle
    Lane B: mouse Brain

References for Anti-S6K1 antibody (ab9366)

This product has been referenced in:
  • Curtis RV  et al. Decline in mammary translational capacity during intravenous glucose infusion into lactating dairy cows. J Dairy Sci 97:430-8 (2014). WB ; Cow . Read more (PubMed: 24268408) »
  • Zhang J  et al. Phosphorylation and degradation of S6K1 (p70S6K1) in response to persistent JNK1 Activation. Biochim Biophys Acta 1832:1980-8 (2013). WB . Read more (PubMed: 23816567) »

See all 5 Publications for this product

Product Wall

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Yes the customer would be eligible for a testing discount for ab12864 if they are willing to test it in mouse and/or sheep samples with western blotting. The sequence surrounding the Serine 235 and 236 of human RP...

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Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen. Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten. Ich habe gesehen, dass Sie schon mit meiner Kollegin be...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Liver)
Loading amount 30 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted May 02 2012

Thank you for providing those details.


I can now confirm the discount code is xxxxx for which the expiration date is the 24th of July 2012. In order to activate the code, please submit an Abreview for ab12864 using mouse and/or sheep sa...

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Thank you for getting back to me.

I understand your concerns over the antibody. I suggested that the antibody may be at fault not because we have had other complaints from customers but because of the optimisation having been performed by the...

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Thank you for taking time to complete our questionnaire and for providing me with that extra information. I am sorry that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and w...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 13 2012

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. We take customer complaints very seriously and investigate every antibody we feel may not be performing as expected. T...

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Thank you very much for your interest in ab9366.

To our knowledge, ab9366 has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab9366 now, test it in IHC and submit feedback to us in the form of an A...

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