Anti-S6K1 (phospho T229) antibody (ab5231)
Key features and details
- Rabbit polyclonal to S6K1 (phospho T229)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-S6K1 (phospho T229) antibody
See all S6K1 primary antibodies -
Description
Rabbit polyclonal to S6K1 (phospho T229) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human S6K1 (phospho T229). The sequence is conserved in rat.
-
Positive control
- Hek293 cells +/- EGF; truncated p70S6K protein, an N-terminus His-tagged fusion protein corresponding to amino acids 1-421 of human p70S6K containing a T412E mutation to become constitutively active.
-
General notes
70 kDa ribosomal protein S6 kinase (p70S6K or p70S6K beta 1) is a member of a serine/threonine kinase family that phosphorylates the 40S ribosomal protein S6, thereby modulating the translation of ribosomal proteins and translation elongation factors. p70S6K is activated in response to the phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinase (MAPK) pathways, and is required for progression through the cell cycle and for cell growth. Activation of p70S6K is regulated by phosphorylation of seven different residues distributed throughout the protein, with the critical sites being threonines 229, 389, 421 and serine 424. Threonine 229 is located within the activation loop.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p70S6K. The final product is generated by affinity chromatography using a p70S6K derived peptide that is phosphorylated at threonine 229. -
Primary antibody notes
70 kDa ribosomal protein S6 kinase (p70S6K or p70S6K beta 1) is a member of a serine/threonine kinase family that phosphorylates the 40S ribosomal protein S6, thereby modulating the translation of ribosomal proteins and translation elongation factors. p70S6K is activated in response to the phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinase (MAPK) pathways, and is required for progression through the cell cycle and for cell growth. Activation of p70S6K is regulated by phosphorylation of seven different residues distributed throughout the protein, with the critical sites being threonines 229, 389, 421 and serine 424. Threonine 229 is located within the activation loop. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 70 kDa.
|
Notes |
---|
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 70 kDa. |
Target
-
Function
Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
Domain
The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1. -
Post-translational
modificationsPhosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism. -
Cellular localization
Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6198 Human
- Omim: 608938 Human
- SwissProt: P23443 Human
- Unigene: 463642 Human
-
Alternative names
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
see all
Images
-
Extracts prepared from Hek293 cells (1-4) or activated p70 S6 kinase protein (5, 6) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either untreated (1-5), or treated with lambda (ë) phosphatase (6), blocked with a 5% BSA-TBST buffer overnight at 4°C, then incubated with 0.50
µ g/mL ab5231 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: nopeptide (1, 5, 6), the non-phosphorylated peptide corresponding to the phosphopeptide (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4).After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected using the Tropix WesternStar method.The data show that only the phosphopeptide corresponding to p70S6K [pT229] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the si
Datasheets and documents
-
Datasheet download
References (14)
ab5231 has been referenced in 14 publications.
- Jiang Q et al. S6K1-mediated phosphorylation of PDK1 impairs AKT kinase activity and oncogenic functions. Nat Commun 13:1548 (2022). PubMed: 35318320
- Zhou X et al. PI3K inhibition sensitizes EGFR wild-type NSCLC cell lines to erlotinib chemotherapy. Exp Ther Med 21:9 (2021). PubMed: 33235618
- Chen S et al. Salidroside protects SH-SY5Y from pathogenic a-synuclein by promoting cell autophagy via mediation of mTOR/p70S6K signaling. Mol Med Rep 20:529-538 (2019). PubMed: 31180515
- Arif A et al. Multisite Phosphorylation of S6K1 Directs a Kinase Phospho-code that Determines Substrate Selection. Mol Cell 73:446-457.e6 (2019). PubMed: 30612880
- Silva RAC et al. Cardiac Remodeling Induced by All-Trans Retinoic Acid is Detrimental in Normal Rats. Cell Physiol Biochem 43:1449-1459 (2017). PubMed: 29017174