sICAM2 Human ELISA Kit (ab46080)

Overview

• Product namesICAM2 Human ELISA KitSee all ICAM2 kits ...
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  A	8	23.62U/ml	0.277	1.17%
  B	8	2.96U/ml	0.86	2.9%

  Inter-assay

  Sample	n	Mean	SD	CV%
  A	33	23.26U/ml	0.668	2.87%
  B	33	3.12	0.218	6.98%

• Tests
  1 x 96 well plate
• Sample type
  Cell culture supernatant, Serum, Plasma
• Assay typeSandwich
• Sensitivity
  = 0.2 U/ml
• Range
  0.75 U/ml - 24 U/ml
• Assay time
  1h 40m
• Species reactivity
  Reacts with: Human
  
• Product overview

  The Human sICAM-2 ELISA is to be used for the in-vitro quantitative determination of soluble Inter Cellular Adhesion Molecule -2 (sICAM-2) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human sICAM-2. This kit has been configured for research use only and is not to be used in diagnostic procedures.

  PRINCIPLE OF THE METHOD

  The sICAM-2 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for ICAM-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known sICAM-2 concentrations, control specimens and unknowns are pipetted into these wells.

  During the first incubation, the sICAM-2 antigen and a biotinylated monoclonal antibody specific for ICAM-2 are simultaneously incubated.

  After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of sICAM-2 present in the samples.

   

  Recognizes both natural and recombinant human ICAM2
  
  Range: 0.75 U/ml - 24 U/ml
  
  Sensitivity <0.2 U/ml
  

• Notes

  Safety Information:

  • For research use only.
  • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
  • Avoid any skin contact with H₂SO₄ and TMB. In case of contact, wash thoroughly with water.
  • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
  • Do not pipette by mouth.
  • The stop solution in this kit contains 9.8% sulphuric acid.

• Tested applicationsELISA more details

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	Identifier	1 x 96 tests
  12 strips		8 units
  Biotinylated Antibody Diluent	Red	1 x 7.5ml
  Biotinylated anti-sICAM-2	Red	1 x 0.4ml
  Chromogen TMB		1 x 11ml
  Control	Silver	2 vials
  H2SO4: Stop Reagent	Black	1 x 11ml
  HRP Diluent	Red	1 x 23ml
  Standard	Yellow	2 vials
  Standard Diluent: Buffer.x10 Concentrated	Black	1 x 25ml
  Streptavidin-HRP		2 x 5µl
  Washing Buffer.x200 Concentrated	White	1 x 10ml

• Research Areas
  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • T Lymphocytic Lineage

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Adhesion molecules ELISA kits

  • Immunology
  • Cell Type Markers
  • CD
  • Adhesion

Applications

sICAM2 Human ELISA Kit images

• 

  ELISA - Human sICAM2 ELISA Kit (ab46080)
  Typical sICAM-2 standard curve ranging from 0.75 to 24 U/mL

Protocols

• Human sICAM2 ELISA Kit