Anti-SA1 antibody (ab4455)
Key features and details
- Goat polyclonal to SA1
- Suitable for: IP, WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SA1 antibody
See all SA1 primary antibodies -
Description
Goat polyclonal to SA1 -
Host species
Goat -
Tested applications
Suitable for: IP, WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rhesus monkey -
Immunogen
Synthetic peptide within Human SA1 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_005853.2 (GeneID 10274).
Database link: Q8WVM7 -
Positive control
- WB: HeLa nuclear extract. HeLa, MCF-7, HEK-293T, TCMK-1 and NIH/3T3 whole cell lysate. ICC: HeLa cells. IP: SA1 IP in HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
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ICC |
1/500 - 1/2000.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa). |
ICC
1/500 - 1/2000. |
Target
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Function
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. -
Sequence similarities
Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain. -
Post-translational
modificationsPhosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. - Information by UniProt
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Database links
- Entrez Gene: 10274 Human
- Entrez Gene: 20842 Mouse
- Omim: 604358 Human
- SwissProt: Q8WVM7 Human
- SwissProt: Q9D3E6 Mouse
- Unigene: 412586 Human
- Unigene: 42135 Mouse
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Alternative names
- Cohesin subunit SA 1 antibody
- Cohesin subunit SA-1 antibody
- Cohesin Subunit SA1 antibody
see all
Images
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Whole cell lysate (15 µg) from HeLa, MCF-7, HEK-293T, TCMK-1, and NIH/3T3 cells prepared using NETN lysis buffer.
ab4455 used for WB at 0.1 µg/ml.
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Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: ab4455 used for IP at 6 µg per reaction.
Lane 2: Control IgG.
For blotting immunoprecipitated SA1, ab4455 was used at 0.1 µg/ml.
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Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4455 at 0.5
µ g/ml.Detection: ECL with 15 second exposure.
Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4455 at 0.5 µg/ml.
Detection: ECL with 15 second exposure. -
Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4455 at 1µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab4455 has been referenced in 4 publications.
- Tian H et al. EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis protein REC8. Mol Biol Cell 32:1-14 (2021). PubMed: 33175657
- Hopkins J et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet 10:e1004413 (2014). WB ; Mouse . PubMed: 24992337
- Xiao T et al. Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity. Mol Cell Biol 31:2174-83 (2011). WB ; Human . PubMed: 21444719
- Leman AR et al. Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion. J Cell Sci 123:660-70 (2010). WB, IP ; Human . PubMed: 20124417