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Western blot - SA1 antibody (ab4455)
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Immunocytochemistry/ Immunofluorescence - SA1 antibody (ab4455)
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Product Name
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SA1 antibody
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See all SA1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to SA1
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Immunogen
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Synthetic peptide (Human) - 36 amino acids which represent a portion of SA 1 encoded in part by exons 5 and 6.
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Reacts with
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Hu, Xl
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Tested applications
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ICC/IF, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC: 1/500 - 1/2000.
ICC/IF: 1/500.
IP: Use at an assay dependent dilution. (PMID 20124417)
WB: 1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nucleus. Associates with chromatin.
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Research areas
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Chromatin and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Chromatid Cohesion
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
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Relevance
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SA1 is a component of the multisubunit cohesin complex. Cohesin functions to hold sister chromatids together during meiosis and mitosis and ensure proper segregation of chromosomes. SA1 has also been identified as a mediator of p53 induced apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SA1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SA1 antibody images, references, products related to ab4455 and other tools.
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SA1 antibody images:
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 Western blot - SA1 antibody (ab4455)
Predicted band size : 144 kDa
Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4455 at 0.5 µg/ml.
Detection: ECL with 15 second exposure.
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Immunocytochemistry/ Immunofluorescence - SA1 antibody (ab4455)
Goat polyclonal to SA1 antibody (ab4455) at a 1/500 dilution detecting Stromal Antigen 1 in assynchronous HeLa cells. Cells were fixed with 4% paraformaldehyde and counterstained with DAPI. Secondary antibody:Donkey anti-goat IgG conjugated to Cy3. For further information please refer to abreview. This image is courtesy of an Abreview submitted by Dr Kirk McManus
See Abreview
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References for SA1 antibody (ab4455)
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This product has been used in:
Leman AR et al. Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion. J Cell Sci 123:660-70 (2010). WB, IP; Human. PubMed: 20124417
If you publish research using ab4455 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to SA1
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SA1 antibody - more information
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Customer reviews (feedback) regarding SA1 antibody |
Customer FAQs regarding SA1 antibody |
Protocols for SA1 antibody |
Price and availability of products related to SA1 antibody |
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SA1 antibody - related products:
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SA1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about SA1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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