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Western blot - SA1 antibody - ChIP Grade (ab4457)
(enlarge)
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Product Name
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SA1 antibody - ChIP Grade
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See all SA1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to SA1 - ChIP Grade
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Immunogen
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Synthetic peptide (Human) - 35 amino acids which represented a portion of SA 1 encoded in part by exons 35 and 36.
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Reacts with
(species key)
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Hu, Xl
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Epitope
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The epitope recognized by ab4457 maps to a region between residues 1150
and 1200 of human Stromal Antigen 1 using the numbering given in entry
NP_005853.2 (GeneID 10274).
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Tested applications
(see key)
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ChIP, ChIP/Chip, ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 18550811).
ChIP/Chip: Use at an assay dependent dilution (PMID 18550811).
ICC: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nucleus. Associates with chromatin.
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Chromatid Cohesion
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
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Relevance
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SA1 is a component of the multisubunit cohesin complex. Cohesin functions to hold sister chromatids together during meiosis and mitosis and ensure proper segregation of chromosomes. SA1 has also been identified as a mediator of p53 induced apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SA1 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SA1 antibody - ChIP Grade images, references, products related to ab4457 and other tools.
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SA1 antibody - ChIP Grade images:
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 Western blot - SA1 antibody - ChIP Grade (ab4457)
Predicted band size : 144 kDa
Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4457 at 0.5 µg/ml.
Detection: ECL with 15 second exposure.
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References for SA1 antibody - ChIP Grade (ab4457)
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This product has been used in: (two most recent references)
Schmidt D et al. A CTCF-independent role for cohesin in tissue-specific transcription. Genome Res 20:578-88 (2010). ChIP; Human. PubMed: 20219941
Rubio ED et al. CTCF physically links cohesin to chromatin. Proc Natl Acad Sci U S A 105:8309-14 (2008). ChIP, ChIP/Chip; Human. PubMed: 18550811
If you publish research using ab4457 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to SA1
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SA1 antibody - ChIP Grade - more information
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Customer reviews (feedback) regarding SA1 antibody - ChIP Grade |
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SA1 antibody - ChIP Grade - related products:
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SA1 antibody - ChIP Grade - other tools:
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Contact Abcam with a Technical Enquiry about SA1 antibody - ChIP Grade |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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