Overview

  • Product nameAnti-SA1 antibody - ChIP Grade
    See all SA1 primary antibodies
  • Description
    Goat polyclonal to SA1 - ChIP Grade
  • Tested applicationsSuitable for: WB, ICC, ChIP, ChIP/Chipmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human SA1 aa 1150-1200.

Properties

Applications

Our Abpromise guarantee covers the use of ab4457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
ICC 1/500 - 1/2000.
ChIP Use at an assay dependent concentration. PubMed: 18550811
ChIP/Chip Use at an assay dependent concentration. PubMed: 18550811

Target

  • FunctionComponent of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
  • Sequence similaritiesBelongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.
  • Post-translational
    modifications
    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
  • Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cohesin subunit SA 1 antibody
    • Cohesin subunit SA-1 antibody
    • Cohesin Subunit SA1 antibody
    • Nuclear protein stromal antigen 1 antibody
    • SA 1 antibody
    • SCC 3 homolog 1 antibody
    • Scc3 antibody
    • SCC3 homolog 1 antibody
    • STAG 1 antibody
    • stag1 antibody
    • STAG1_HUMAN antibody
    • Stromal antigen 1 antibody
    see all

Anti-SA1 antibody - ChIP Grade images

  • Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4457 at 1µg/ml.


  • Predicted band size : 144 kDa

    Nuclear Extract from HeLa cells (~5 x 105 cells).

    ab4457 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.

    Nuclear Extract from HeLa cells (~5 x 105 cells).

    ab4457 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.

References for Anti-SA1 antibody - ChIP Grade (ab4457)

This product has been referenced in:
  • Uusküla-Reimand L  et al. Topoisomerase II beta interacts with cohesin and CTCF at topological domain borders. Genome Biol 17:182 (2016). WB . Read more (PubMed: 27582050) »
  • Faure AJ  et al. Cohesin regulates tissue-specific expression by stabilizing highly occupied cis-regulatory modules. Genome Res 22:2163-75 (2012). CHIPseq ; Mouse . Read more (PubMed: 22780989) »

See all 4 Publications for this product

Product Wall

The epitope recognized by ab4457 maps to a region between residues 1150 and 1200 of human Stromal Antigen 1 using the numbering given in entry NP_005853.2 (GeneID 10274). I hope this helps, please contact us again if you have any additional questions.

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Thank you very much for your patience. I'm afraid that I am unable to get this information from the originator of ab4455 and ab4457. I apologize for the inconvenience and if I can be of further assistance, please do let me know.

Thank you for your email. All the information that we have regarding these antibodies is located on the online datasheets. I unfortunately can't tell you which one works "best." Are you detecting SA1 or SA2? The differences between the two SA1 antibodi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"