Anti-SA2 antibody - C-terminal (ab229609)
Key features and details
- Rabbit polyclonal to SA2 - C-terminal
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SA2 antibody - C-terminal
See all SA2 primary antibodies -
Description
Rabbit polyclonal to SA2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SA2 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated
Database link: Q8N3U4 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HeLa whole cell extract. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/10000. Predicted molecular weight: 141 kDa.
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IP |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/10000. Predicted molecular weight: 141 kDa. |
IP
1/100 - 1/500. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. -
Sequence similarities
Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain. -
Post-translational
modificationsPhosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. - Information by UniProt
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Database links
- Entrez Gene: 10735 Human
- Omim: 300826 Human
- SwissProt: Q8N3U4 Human
- Unigene: 496710 Human
- Unigene: 624663 Human
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Alternative names
- bA517O1.1 antibody
- Cohesin Subunit SA 2 antibody
- Cohesin subunit SA-2 antibody
see all
Images
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All lanes : Anti-SA2 antibody - C-terminal (ab229609) at 1/6000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : SA2 shRNA-transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 141 kDa5% SDS-PAGE gel.
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4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SA2 (green) using ab229609 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). F-actin stained with Phalloidin (red).
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All lanes : Anti-SA2 antibody - C-terminal (ab229609) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 141 kDa5% SDS-PAGE gel.
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SA2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5μg ab229609. Western blot was performed from the immunoprecipitate using ab229609.
Lane 1: Control IgG instead of ab229609 in HeLa whole cell extract.
Lane 2: ab229609 IP in HeLa whole cell extract.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab229609 has been referenced in 1 publication.
- Li JT et al. Dietary folate drives methionine metabolism to promote cancer development by stabilizing MAT IIA. Signal Transduct Target Ther 7:192 (2022). PubMed: 35729157