Overview

  • Product name
    Anti-SAC3D1 antibody
  • Description
    Rabbit polyclonal to SAC3D1
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 268-376 of Human SAC3D1.

  • Positive control
    • Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Relevance
    Involved in centrosome duplication and mitotic progression.
  • Cellular localization
    Cytoplasm; cytoskeleton; centrosome By similarity. Cytoplasm; cytoskeleton; spindle By similarity. Note: Localizes on centrosomes in interphase cells and at spindles in mitosis
  • Database links
  • Alternative names
    • HSU79266 antibody
    • SAC3 domain-containing protein 1 antibody
    • SAC3 homology domain-containing protein 1 antibody
    • SHD1 antibody

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122809 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122809, at 1/200 dilution, staining SAC3D1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

References

ab122809 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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