Purification notesab71959 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).
Use at an assay dependent dilution.
FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Tissue specificityExpression level increases during S phase and drops in G2 phase (at protein level).
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.