Overview

  • Product nameAnti-SAE1 antibody [2946C4a]
    See all SAE1 primary antibodies
  • Description
    Mouse monoclonal [2946C4a] to SAE1
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of human SAE1

  • Positive control
    • Recombinant human SAE1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4)
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab71959 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • ClonalityMonoclonal
  • Clone number2946C4a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • Tissue specificityExpression level increases during S phase and drops in G2 phase (at protein level).
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator of SUMO1 antibody
    • AOS1 antibody
    • HSPC140 antibody
    • Sae1 antibody
    • SAE1_HUMAN antibody
    • Sentrin/SUMO activating protein AOS1 antibody
    • SUA1 antibody
    • SUMO 1 activating enzyme E1 N subunit antibody
    • SUMO 1 activating enzyme subunit 1 antibody
    • SUMO-activating enzyme subunit 1 antibody
    • Ubiquitin like protein SUMO1 activating enzyme antibody
    • Ubiquitin-like 1-activating enzyme E1A antibody
    • UBL E1A antibody
    • UBLE1A antibody
    see all

Anti-SAE1 antibody [2946C4a] images

References for Anti-SAE1 antibody [2946C4a] (ab71959)

ab71959 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"