The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notesIs unsuitable for WB.
FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Tissue specificityExpression level increases during S phase and drops in G2 phase (at protein level).
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitin like protein SUMO1 activating enzyme antibody
Ubiquitin-like 1-activating enzyme E1A antibody
UBL E1A antibody
Anti-SAE1 antibody images
Immunoprecipitation - SAE1 antibody (ab103522)
Detection of SAE1 by Western Blot of Immunprecipitate.
ab103522 at 0.4µg/ml staining SAE1 in HeLa whole cell lysate immunoprecipitated using ab103522 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.