Overview

  • Product nameAnti-SAE1 antibody
    See all SAE1 primary antibodies
  • Description
    Rabbit polyclonal to SAE1
  • Tested applicationsSuitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 296-346 of Human SAE1 (NP_005491.1).

  • Positive control
    • HeLa whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab103522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
    • Tissue specificityExpression level increases during S phase and drops in G2 phase (at protein level).
    • PathwayProtein modification; protein sumoylation.
    • Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator of SUMO1 antibody
      • AOS1 antibody
      • HSPC140 antibody
      • Sae1 antibody
      • SAE1_HUMAN antibody
      • Sentrin/SUMO activating protein AOS1 antibody
      • SUA1 antibody
      • SUMO 1 activating enzyme E1 N subunit antibody
      • SUMO 1 activating enzyme subunit 1 antibody
      • SUMO-activating enzyme subunit 1 antibody
      • Ubiquitin like protein SUMO1 activating enzyme antibody
      • Ubiquitin-like 1-activating enzyme E1A antibody
      • UBL E1A antibody
      • UBLE1A antibody
      see all

    Anti-SAE1 antibody images

    • Detection of SAE1 by Western Blot of Immunprecipitate.
      ab103522 at 0.4µg/ml staining SAE1 in HeLa whole cell lysate immunoprecipitated using ab103522 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SAE1 with ab103522 at 1/1000 (1µg/ml). Detection: DAB.

    References for Anti-SAE1 antibody (ab103522)

    ab103522 has not yet been referenced specifically in any publications.

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