Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab5645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Tested in peptide ELISA: antibody detection limit dilution 1/8000.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
    • Tissue specificity
      Expression level increases during S phase and drops in G2 phase (at protein level).
    • Pathway
      Protein modification; protein sumoylation.
    • Sequence similarities
      Belongs to the ubiquitin-activating E1 family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator of SUMO1 antibody
      • AOS1 antibody
      • HSPC140 antibody
      • Sae1 antibody
      • SAE1_HUMAN antibody
      • Sentrin/SUMO activating protein AOS1 antibody
      • SUA1 antibody
      • SUMO 1 activating enzyme E1 N subunit antibody
      • SUMO 1 activating enzyme subunit 1 antibody
      • SUMO-activating enzyme subunit 1 antibody
      • Ubiquitin like protein SUMO1 activating enzyme antibody
      • Ubiquitin-like 1-activating enzyme E1A antibody
      • UBL E1A antibody
      • UBLE1A antibody
      see all

    Images



    • Predicted band size : 40 kDa


      ab5645 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References

    This product has been referenced in:
    • Jones MC  et al. Regulation of the SUMO pathway sensitizes differentiating human endometrial stromal cells to progesterone. Proc Natl Acad Sci U S A 103:16272-7 (2006). Read more (PubMed: 17053081) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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